The methyl-5 alpha-dihydrotestosterones mesterolone and drostanolone; gas chromatographic/mass spectrometric characterization of the urinary metabolites

The methyl-5 alpha-dihydrotestosterones mesterolone and drostanolone; gas chromatographic/mass spectrometric characterization of the urinary metabolites / Douwe de Boer, E.G. de Jong, R.A. Maes, J.M. van Rossum. - (Journal of Steroid Biochemistry and Molecular Biology 42 (1992) 3-4 (May); p. 411-419)

  • PMID: 1606052
  • DOI: 10.1016/0960-0760(92)90146-a


Abstract

Before including the detection of the methyl-5 alpha-dihydrotestosterones mesterolone (1 alpha-methyl-17 beta-hydroxy-5 alpha-androstan-3-one) and drostanolone (2 alpha-methyl-17 beta-hydroxy-5 alpha-androstan-3-one) in doping control procedures, their urinary metabolites were characterized by gas chromatography/mass spectrometry. Several metabolites were found after enzymatic hydrolysis and conversion of the respective metabolites to their trimethylsilyl-enol-trimethylsilyl ether derivatives. The major metabolites of mesterolone and drostanolone were identified as 1 alpha-methyl-androsterone and 2 alpha-methyl-androsterone, respectively. The parent compounds and the intermediate 3 alpha,17 beta-dihydroxysteroid metabolites were detected as well. The reduction into the corresponding 3 beta-hydroxysteroids was a minor metabolic pathway. All metabolites were found to be conjugated to glucuronic acid.

Parameters

Science
Study
Date
1 May 1992
People
Boer, Douwe de
Maes, Robert A.A.
Rossum, J.M. van
Country
Netherlands
Language
English
Other organisations
Nederlands Instituut voor Drugs- en Doping Research (NIDDR) - Netherlands Institute for Drugs and Doping Research
Analytical aspects
Mass spectrometry analysis
Testing method development
Doping classes
S1. Anabolic Agents
Substances
Drostanolone
Mesterolone
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26 June 2014
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21 November 2020
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