Development of two complementary LC-HRMS methods for analyzing sotatercept in dried blood spots for doping controls

Development of two complementary LC-HRMS methods for analyzing sotatercept in dried blood spots for doping controls / Tobias Lange, Katja Walpurgis, Andreas Thomas, Hans Geyer, Mario Thevis (Bioanalysis 11 (2019) 10)

  • PMID: 31218901
  • DOI: 10.4155/bio-2018-0313


sotatercept is a therapeutic Fc-fusion protein with erythropoiesis-stimulating activity. Due to a potential abuse of the drug by athletes in professional sports, a sensitive detection method is required. In sports drug testing, alternative matrices such as dried blood spots (DBS) are gaining increasing attention as they can provide several advantages over conventional matrices.

Materials & methods:
Herein, two complementary LC-high-resolution mass spectrometry (HRMS) detection methods for sotatercept from DBS, an initial testing procedure (ITP) and a confirmation procedure (CP) were developed and validated for the first time. Both methods comprise an ultrasonication-assisted extraction, affinity enrichment, proteolytic digestion and HRMS detection.

Results & conclusion:
For the multianalyte ITP, artificial samples fortified with sotatercept, luspatercept and bimagrumab, and authentic specimens containing bimagrumab were successfully analyzed as proof-of-concept. The validated detection methods for sotatercept are fit for purpose and the ITP was shown to be suitable for the detection of novel IgG-based pharmaceuticals in doping control DBS samples.


Research / Study
20 June 2019
Geyer, Hans
Lange, Tobias
Thevis, Mario
Thomas, Andreas
Walpurgis, Katja
Other organisations
Deutsche Sporthochschule Köln (DSHS) - German Sport University Cologne
European Monitoring Center for Emerging Doping Agents (EuMoCEDA)
Cologne, Germany: Institute of Biochemistry - German Sport University Cologne
Analytical aspects
Blood testing method
Mass spectrometry analysis
Testing method development
Doping classes
S2. Peptide Hormones, Growth Factors
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Date generated
5 June 2019
Date of last modification
7 September 2020
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