Identification of S22 (ostarine) in human nails and hair using LC-HRMS. Application to two authentic cases

Identification of S22 (ostarine) in human nails and hair using LC-HRMS : Application to two authentic cases / Pascal Kintz, Laurie Gheddar, Alice Ameline, Jean-Sébastien Raul. - (Drug Testing and Analysis (2020) 20 July)

  • PMID: 32688441
  • DOI: 10.1002/dta.2902


Ostarine, also known as S22 or MK2866 and enobosarm, is a selective androgen receptor modulator (SARM). It has high anabolic potency, in addition to limited androgenic effects. At this time, ostarine has no therapeutic use, but can be abused for performance-enhancing purposes using the oral route, at dosages of 10-25 mg per day. As the drug can easily be obtained via the Internet or some fitness centers, athletes and more and more amateurs can use it without undergoing the deleterious physiological side effects that are generally associated with testosterone-related compounds. Since 2008, the World Anti-Doping Agency (WADA) has prohibited SARMs at-all-times in the category of "other anabolic agents" under section S1.2 of the WADA List. In a case of trafficking/abuse, ostarine was identified in nail (subject 1) and hair (subject 2) by LC/HRMS after incubation of 50 mg of matrix in a pH 9.5 buffer, followed by extraction with organic solvents. The drug was quantitated by LC-MS/MS. Ostarine tested positive at 61 pg/mg (toenails) and 111 pg/mg (fingernails) for subject 1. Ostarine was present at 146, 168, 93, and 101 pg/mg in the 4 × 3 cm hair sections of subject 2, clearly demonstrating long-term use.


20 July 2020
Ameline, Alice
Gheddar, Laurie
Kintz, Pascal
Raul, Jean-Sébastien
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Institut de médecine légale de Strasbourg
Université de Strasbourg (Unistra) - University of Strasbourg
Analytical aspects
Mass spectrometry analysis
Testing method development
Doping classes
S1. Anabolic Agents
Enobosarm (ostarine)
Selective androgen receptor modulators (SARMs)
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7 September 2020
Date of last modification
13 September 2020
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