Identification of a novel growth hormone releasing peptide (a glycine analogue of GHRP-2) in a seized injection vial

Identification of a novel growth hormone releasing peptide (a glycine analogue of GHRP-2) in a seized injection vial / Magdalena Popławska, Agata Błażewicz. - (Drug Testing and Analysis 11 (2019) 1 (January); p. 162-167)

  • PMID: 30051972
  • DOI: 10.1002/dta.2467


Abstract

Growth hormone releasing peptides (GHRPs) are synthetic peptides with the ability to stimulate human growth hormone (hGH) secretion. Several GHRPs have been developed as drug candidates; however, only one of them, GHRP-2 (Pralmorelin), has received a clinical approval. Nevertheless, they are distributed on the black market and misused by cheating athletes, due to their performance-enhancing effects. Hence, GHRPs have been included in the World-Anti-Doping-Agency's Prohibited List as forbidden substances in sport. Predominantly, analytical methods for detection and unequivocal identification of doping substances are based on mass spectrometry. Therefore, in the present work, a qualitative analysis by liquid chromatography coupled to high-resolution tandem mass spectrometry with a quadrupole time-of-flight analyzer was performed to identify a new heptapeptide (MW = 874.02 Da) - a glycine analogue of GHRP-2. Structure determination using de novo sequencing is described here in detail. The results of this study may indicate a new approach to circumvent a detection of doping practices.

Parameters

Science
Study
Date
27 July 2018
People
Błażewicz. Agata
Poplawska, Magdalena
Country
Poland
Language
English
Other organisations
Narodowy Instytut Leków, Warszawa - National Medicines Institute, Warsaw
Analytical aspects
Mass spectrometry analysis
Doping classes
S2. Peptide Hormones, Growth Factors
Substances
Growth hormone (GH)
Pralmorelin (GHRP-2)
Various
Illegal production / trade
Document category
Abstract
Date generated
23 September 2020
Date of last modification
24 September 2020
Category
  • Legal Source
  • Education
  • Science
  • Statistics
  • History
Country & language
  • Country
  • Language
Other filters
  • ADRV
  • Legal Terms
  • Sport/IFs
  • Other organisations
  • Laboratories
  • Analytical aspects
  • Doping classes
  • Substances
  • Medical terms
  • Various
  • Version
  • Document category
  • Document type
Publication period
Origin