The detection of danazol and its significance in doping analysis

The detection of danazol and its significance in doping analysis / Douwe de Boer, E.G. de Jong, R.A. Maes. - (Journal of Analytical Toxicology 16 (1992) 1 (January-February); p. 14-18)

  • PMID: 1640693
  • DOI: 10.1093/jat/16.1.14


The use of anabolic steroids and related compounds in sport is forbidden by the International Olympic Committee (IOC). Because danazol (17 alpha-pregna-2,4-dien-20-yno[2,3-D] isoxazol-17 beta-ol) is structurally related to the anabolic steroid stanozolol, its use should be questioned. Therefore, the detection and the significance of danazol in doping analysis are discussed. A urine specimen suspected of containing danazol metabolites was analyzed in order to characterize the metabolites. After isolation and conversion into three different derivatives, the metabolites were subjected to gas chromatography/mass spectrometry (GC/MS) in the electron impact (EI) mode. The structure assignment was based on the molecular ions, fragmentation patterns observed for the three different derivatives, and the possible metabolite structures given in the literature. Ethisterone was identified as a nonconjugated metabolite. 2-Hydroxymethylethisterone was observed in two stereoisomeric forms. One stereoisomer was found mainly in the nonconjugated steroid fraction and the other in the conjugated fraction. The results were confirmed by analyzing urine specimens of a volunteer who was known to have taken danazol. Derivatization methods and GC/MS data are given to implement danazol detection in routine screening and confirmation procedures.


Research / Study
1 January 1992
Boer, Douwe de
Jong, Edward G. de
Maes, Robert A.A.
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Nederlands Instituut voor Drugs- en Doping Research (NIDDR) - Netherlands Institute for Drugs and Doping Research
Analytical aspects
Mass spectrometry analysis
Testing method development
Doping classes
S1. Anabolic Agents
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Date generated
19 November 2020
Date of last modification
23 November 2020
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