The analysis of trenbolone and the human urinary metabolites of trenbolone acetate by gas chromatography/mass spectrometry and gas chromatography/tandem mass spectrometry

The analysis of trenbolone and the human urinary metabolites of trenbolone acetate by gas chromatography/mass spectrometry and gas chromatography/tandem mass spectrometry / Douwe de Boer, M.E. Gainza Bernal, R.D. van Ooyen, R.A. Maes. - (Biological Mass Spectromety 20 (1991) 8 (August); p. 459-466)

  • PMID: 1768702
  • DOI: 10.1002/bms.1200200805


Abstract

The electron impact mass spectrometric properties of trimethylsilyl ether and fluoroacyl ester derivatives of trenbolone, combined or not combined with a methoxime group, are presented. Some derivatization problems were observed and were due to the formation of enol derivatives at the 3C-position in several tautomeric forms, which in their turn were not stable and lost two or four hydrogens under the conditions studied. The enolization could be minimized by carefully selecting the reaction conditions or could be prevented by the introduction of a methoxime group at the 3C-position. The limits of detection and identification of the methoxime heptafluorobutyryl ester and the methoxime trimethylsilyl ether derivative of trenbolone were determined using a mass selective detector in the electron impact mode and a triple-stage quadrupole in the methane positive chemical ionization mode. Selected reaction monitoring in tandem mass spectrometry did not improve the limit of detection, but because of the gain in selectivity did improve the limit of identification. The glucuronides of trenbolone and epitrenbolone could be identified in three urine specimens out of 200 samples in routine doping control.

Parameters

Science
Study
Date
1 August 1991
People
Boer, Douwe de
Gainza Bernal, M.E.
Maes, Robert A.A.
Ooyen, R.D. van
Country
Netherlands
Language
English
Other organisations
Nederlands Instituut voor Drugs- en Doping Research (NIDDR) - Netherlands Institute for Drugs and Doping Research
Analytical aspects
Mass spectrometry analysis
Testing method development
Doping classes
S1. Anabolic Agents
Substances
Trenbolone (17β-hydroxyestr-4,9,11-trien-3-one)
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19 November 2020
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21 November 2020
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