Detection of anabolic steroids in head hair

Detection of anabolic steroids in head hair / Xin-Sheng Deng, Akira Kurosu, Derrick J. Pounder. - (Journal of forensic sciences 44 (1999) 2 (March); p. 343-346)

  • PMID: 10097359
  • DOI: 10.1520/JFS14460J


Abstract

We developed a gas chromatography/mass spectrometry method for detection and quantitation of anabolic steroids in head hair. Following alkaline digestion and solid-phase extraction, the MO-TMS derivatives gave a specific fragmentation pattern with EI ionization. For stanozolol, the TMS-HFBA derivative showed several diagnostic ions. For androstanolone, mestanolone (methylandrostanolone), and oxymetholone two chromatographic peaks for cis and trans isomers of derivatives were seen. Recoveries were 35 to 45% for androstanolone, oxymetholone, chlorotestosterone-acetate, dehydromethyltestosterone, dehydrotestosterone, fluoxymesterone, mestanolone, methyltestosterone, and nandrolone; 52% for mesterolone, trenbolone; 65% for bolasterone; 24% for methenolone and 17% for stanozolol. Limits of detection were 0.002 to 0.05 ng/mg and of quantitation were 0.02 to 0.1 ng/mg. Seven white male steroid abusers provided head hair samples (10 to 63 mg) and urine. In the hair samples, methyltestosterone was detected in two (confirmed in urine); nandrolone in two (also confirmed in urine); dehydromethyltestosterone in four (but not found in urine); and clenbuterol in one (but not in urine). Oxymethalone was found in urine in one, but not in the hair. One abuser had high levels of testosterone: 0.15 ng/mg hair, and 1190 ng/mL urine. We conclude that head hair analysis has considerable potential for the detection and monitoring of steroid abuse.

Original document

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Science
Study
Date
20 August 1998
People
Deng, Xin-Sheng
Kurosu, Akira
Pounder, Derrick J.
Country
United Kingdom
Language
English
Other organisations
University of Dundee
Analytical aspects
Hairtest
Mass spectrometry analysis
Testing method development
Doping classes
S1. Anabolic Agents
Substances
Clenbuterol
Dehydrochlormethyltestosterone (4-chloro-17β-hydroxy-17α-methylandrosta-1,4-dien-3-one)
Fluoxymesterone
Mestanolone
Methyltestosterone
Nandrolone
Oxymetholone
Stanozolol
Testosterone
Trenbolone (17β-hydroxyestr-4,9,11-trien-3-one)
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3 March 2021
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