Stanozolol-N-glucuronide metabolites in human urine samples as suitable targets in terms of routine anti-doping analysis

Stanozolol-N-glucuronide metabolites in human urine samples as suitable targets in terms of routine anti-doping analysis / Lorenz Göschl, Günter Gmeiner, Peter Gärtner, Georg Stadler, Valentin Enev, Mario Thevis, Wilhelm Schänzer, Sven Guddat, Guro Forsdahl. - (Drug Testing and Analysis (2021) 5 June)

  • PMID: 34089570
  • DOI: 10.1002/dta.3109


Abstract

The exogenous anabolic-androgenic steroid (AAS) stanozolol stays one of the most detected substances in professional sports. Its detection is a fundamental part of doping analysis, and the analysis of this steroid has been intensively investigated for a long time. This contribution to the detection of stanozolol doping describes for the first time the unambiguous proof for the existence of 17-epistanozolol-1'N-glucuronide and 17-epistanozolol-2'N-glucuronide in stanozolol-positive human urine samples due to the access to high-quality reference standards. Examination of excretion study samples shows large detection windows for the phase-II metabolites stanozolol-1'N-glucuronide and 17-epistanozolol-1'N-glucuronide up to 12 days and respectively up to almost 28 days. In addition, we present appropriate validation parameters for the analysis of these metabolites using a fully automatic method online solid-phase extraction (SPE) method already published before. Limits of identification (LOIs) as low as 100 pg/ml and other validation parameters like accuracy, precision, sensitivity, robustness, and linearity are given.

Original document

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Science
Research / Study
Date
5 June 2021
People
Enev, Valentin
Forsdahl, Guro
Gärtner, Peter
Gmeiner, Günter
Göschl, Lorenz
Guddat, Sven
Schänzer, Wilhelm
Stadler, George
Thevis, Mario
Country
Austria
Germany
Norway
Language
English
Other organisations
Deutsche Sporthochschule Köln (DSHS) - German Sport University Cologne
Technische Universität Wien (TUW) - Technical University of Vienna (TU Wien)
Universitetet i Tromsø – Norges arktiske universitet - University of Tromsø - The Arctic University of Norway
Laboratories
Seibersdorf, Austria: Seibersdorf Labor GmbH Doping Control Laboratory
Analytical aspects
Mass spectrometry analysis
Testing method development
Doping classes
S1. Anabolic Agents
Substances
Stanozolol
Document category
Scientific article
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Date generated
15 June 2021
Date of last modification
23 June 2021
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  • Analytical aspects
  • Doping classes
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