Stanozolol-N-glucuronide metabolites in human urine samples as suitable targets in terms of routine anti-doping analysis

Stanozolol-N-glucuronide metabolites in human urine samples as suitable targets in terms of routine anti-doping analysis / Lorenz Göschl, Günter Gmeiner, Peter Gärtner, Georg Stadler, Valentin Enev, Mario Thevis, Wilhelm Schänzer, Sven Guddat, Guro Forsdahl. - (Drug Testing and Analysis (2021) 5 June)

  • PMID: 34089570
  • DOI: 10.1002/dta.3109


The exogenous anabolic-androgenic steroid (AAS) stanozolol stays one of the most detected substances in professional sports. Its detection is a fundamental part of doping analysis, and the analysis of this steroid has been intensively investigated for a long time. This contribution to the detection of stanozolol doping describes for the first time the unambiguous proof for the existence of 17-epistanozolol-1'N-glucuronide and 17-epistanozolol-2'N-glucuronide in stanozolol-positive human urine samples due to the access to high-quality reference standards. Examination of excretion study samples shows large detection windows for the phase-II metabolites stanozolol-1'N-glucuronide and 17-epistanozolol-1'N-glucuronide up to 12 days and respectively up to almost 28 days. In addition, we present appropriate validation parameters for the analysis of these metabolites using a fully automatic method online solid-phase extraction (SPE) method already published before. Limits of identification (LOIs) as low as 100 pg/ml and other validation parameters like accuracy, precision, sensitivity, robustness, and linearity are given.

Original document


Research / Study
5 June 2021
Enev, Valentin
Forsdahl, Guro
Gärtner, Peter
Gmeiner, Günter
Göschl, Lorenz
Guddat, Sven
Schänzer, Wilhelm
Stadler, George
Thevis, Mario
Other organisations
Deutsche Sporthochschule Köln (DSHS) - German Sport University Cologne
Technische Universität Wien (TUW) - Technical University of Vienna (TU Wien)
Universitetet i Tromsø – Norges arktiske universitet - University of Tromsø - The Arctic University of Norway
Seibersdorf, Austria: Seibersdorf Labor GmbH Doping Control Laboratory
Analytical aspects
Mass spectrometry analysis
Testing method development
Doping classes
S1. Anabolic Agents
Document category
Scientific article
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Date generated
15 June 2021
Date of last modification
23 June 2021
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