Untargeted profiling of urinary steroid metabolites after testosterone ingestion: opening new perspectives for antidoping testing

Untargeted profiling of urinary steroid metabolites after testosterone ingestion: opening new perspectives for antidoping testing / Julien Boccard, Flavia Badoud, Nicolas Jan, Raul Nicoli, Carine Schweizer, François Pralong, Jean-Luc Veuthey, Norbert Baume, Serge Rudaz, Martial Saugy. - (Bioanalysis 6 (2014) 19; p. 2523-2536)

  • PMID: 25411696
  • DOI: 10.4155/bio.14.200


Abstract

Aim: Antidoping procedures are expected to greatly benefit from untargeted metabolomic approaches through the discovery of new biomarkers of prohibited substances abuse.

Results: Endogenous steroid metabolites were monitored in urine samples from a controlled elimination study of testosterone undecanoate after ingestion. A platform coupling ultra-high pressure LC with high-resolution quadrupole TOF MS was used and high between-subject metabolic variability was successfully handled using a multiblock data analysis strategy. Links between specific subsets of metabolites and influential genetic polymorphisms of the UGT2B17 enzyme were highlighted.

Conclusion: This exploratory metabolomic strategy constitutes a first step toward a better understanding of the underlying patterns driving the high interindividual variability of steroid metabolism. Promising biomarkers were selected for further targeted study.

Original document

Parameters

Science
Study
Date
20 November 2014
People
Badoud, Flavia
Baume, Norbert
Boccard, Julien
Jan, Nicolas
Nicoli, Raul
Pralong, François
Rudaz, Serge
Saugy, Martial
Schweizer, Carine
Veuthey, Jean-Luc
Country
Switzerland
Language
English
Other organisations
Université de Genève - University of Geneva
Université de Lausanne (UNIL) - University of Lausanne
Laboratories
Lausanne, Switzerland: Laboratoire Suisse d’Analyse du Dopage
Analytical aspects
Mass spectrometry analysis
Testing method development
Doping classes
S1. Anabolic Agents
Substances
Testosterone
Document category
Scientific article
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Pdf file
Date generated
15 July 2021
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  • Legal Source
  • Education
  • Science
  • Statistics
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  • ADRV
  • Legal Terms
  • Sport/IFs
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  • Laboratories
  • Analytical aspects
  • Doping classes
  • Substances
  • Medical terms
  • Various
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