Screening for benfluorex and its major urinary metabolites in routine doping controls

Screening for benfluorex and its major urinary metabolites in routine doping controls / Mario Thevis, Gerd Sigmund, Vassilios Gougoulidis, Simon Beuck, Nils Schlörer, Andreas Thomas, Dorota Kwiatkowska, Andrzej Pokrywka, Wilhelm Schänzer. - (Analytical and Bioanalytical Chemistry 401 (2011) 2 (August); p. 543–551)

  • PMID: 21116611
  • DOI: 10.1007/s00216-010-4455-4


Benfluorex [1-(m-trifluoromethylphenyl)-2-(β-benzoyloxyethyl)aminopropane] has been widely used for the treatment of atherogenic metabolic disorders and impaired carbohydrate metabolism (particularly in obese type-II diabetic patients) as well as an anorectic drug. Due to its potentially performance-enhancing properties, benfluorex has been added to the list of prohibited compounds and methods of doping by the World Anti-Doping Agency (WADA) in 2010, necessitating the implementation of the drug as well as its major metabolites into routine doping control procedures. In the present study, human urinary metabolites of benfluorex were characterized by gas chromatography-electron ionization-mass spectrometry (GC-EI-MS) as well as liquid chromatography-electrospray ionization-high resolution/high accuracy tandem mass spectrometry (LC-ESI-MS/MS). Commonly employed sports drug testing approaches consisting of liquid-liquid extraction followed by GC-MS or urine dilution and immediate LC-MS/MS analysis were expanded and validated with regard to specificity, recovery (48-54%, GC-MS only), intra- and interday precision (<25%), limits of detection (5-8 ng/mL for LC-MS/MS and 80 ng/mL for GC-MS), and ion suppression (for LC-ESI-MS/MS only) to allow the detection of benfluorex metabolites 1-(m-trifluoromethylphenyl)-2-(2-hydroxyethyl)aminopropane (M1), 1-(m-trifluoromethylphenyl)-2-(2-carboxymethyl)aminopropane (M2), and 1-(m-trifluoromethylphenyl)-2-aminopropane (M3) as well as the glucuronic acid conjugate of M1.

Original document


Research / Study
30 November 2010
Beuck, Simon
Gougoulidis, Vassilios
Kwiatkowska, Dorota
Pokrywka, Andrzej
Schänzer, Wilhelm
Schlörer, Nils
Sigmund, Gerd
Thevis, Mario
Thomas, Andreas
Other organisations
Deutsche Sporthochschule Köln (DSHS) - German Sport University Cologne
Instytut Sportu - Państwowy Instytut Badawczy - Polish Institute of Sport
Analytical aspects
Mass spectrometry analysis
Testing method development
Doping classes
S6. Stimulants
Document category
Scientific article
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Pdf file
Date generated
28 July 2021
Date of last modification
29 July 2021
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