Screening for 2-quinolinone-derived selective androgen receptor agonists in doping control analysis

Screening for 2-quinolinone-derived selective androgen receptor agonists in doping control analysis / Mario Thevis, Maxie Kohler, Joachim Maurer, Nils Schlörer, Matthias Kamber, Wilhelm Schänzer. - (Rapid Communicaton in Mass Spectrometry 21 (2007) 21 (15 November); p. 3477-3486)

  • PMID: 17985352
  • DOI: 10.1002/rcm.3247


Abstract

Selective androgen receptor modulators (SARMs) represent a class of emerging drugs with high potential for misuse in sports, and therefore members of this group are banned as anabolic agents by the World Anti-Doping Agency. Preventive approaches to restrict their use include early implementation of target analytes into doping control screening assays and evaluation of the mass spectrometric behavior of these drugs to allow their unequivocal identification as well as the characterization of structurally related compounds and metabolic products. Four model SARMs with the 6-alkylamino-2-quinolinone structure, including the advanced drug candidate LGD-2226, were synthesized. Fragmentation pathways after positive electrospray ionization and collision-induced dissociation were studied using an LTQ Orbitrap mass analyzer, and diagnostic product ions and common dissociation pathways were employed to establish a screening procedure targeting intact quinolinone-based SARMs as well as putative metabolic products such as dealkylated analogues. Therefore, features of a triple quadrupole mass analyzer such as multiple reaction monitoring and precursor ion scanning were utilized. Sample preparation based on commonly employed liquid-liquid extraction and subsequent liquid chromatographic/tandem mass spectrometric measurement allowed for detection limits of 0.01-0.2 ng/mL, and intra- and interday precisions between 3.2 and 8.5% and between 6.3 and 16.6%, respectively. Recoveries varied from 81 to 98%, and tests for ion suppression or enhancement effects were negative for all analytes.

Original document

Parameters

Science
Research / Study
Date
8 October 2007
People
Kamber, Matthias
Kohler, Maxie
Maurer, Joachim
Schänzer, Wilhelm
Schlörer, Nils
Thevis, Mario
Country
Germany
Switzerland
Language
English
Other organisations
Antidoping Switzerland
Deutsche Sporthochschule Köln (DSHS) - German Sport University Cologne
Universität zu Köln - University of Cologne
Analytical aspects
Mass spectrometry analysis
Testing method development
Doping classes
S1. Anabolic Agents
Substances
Selective androgen receptor modulators (SARMs)
Document category
Scientific article
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Pdf file
Date generated
28 July 2021
Date of last modification
29 July 2021
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  • ADRV
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  • Doping classes
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