Annual banned-substance review: analytical approaches in human sports drug testing - [2020-2021]

Annual banned-substance review: analytical approaches in human sports drug testing / Mario Thevis, Tiia Kuuranne, Hans Geyer

  • Drug Testing and Analysis 13 (2021) 17 November
  • PMID: 34788500
  • DOI: 10.1002/dta.3199


  • Introduction
  • Anabolic Agent
    • Anabolic-androgenic steroids
    • Initial testing procedures: Comprehensive screening, metabolism studies
    • Steroid profiling in urine and serum
    • Confirmatory testing procedures – IRMS
    • Other anabolic agents
  • Peptide Hormones, Growth Factors, Related Substances, and Mimetics
    • Erythropoietin-receptor agonists and hypoxia-inducible factor (HIF) activating agents
    • Growth hormone, its fragments and releasing factors, chorionic gonadotrophin and luteinizing hormone (LH)
  • β2‐Agonists
  • Hormone and Metabolic Modulators
  • Diuretics and other Masking Agents, Stimulants
  • Glucocorticoids and cannabinoids
  • Manipulation of blood and blood components
  • Gene Doping
  • Conclusion


Most core areas of anti-doping research exploit and rely on analytical chemistry, applied to studies aiming at further improving the test methods' analytical sensitivity, the assays' comprehensiveness, the interpretation of metabolic profiles and patterns, but also at facilitating the differentiation of natural/endogenous substances from structurally identical but synthetically derived compounds and comprehending the athlete's exposome. Further, a continuously growing number of advantages of complementary matrices such as dried blood spots have been identified and transferred from research to sports drug testing routine applications, with an overall gain of valuable additions to the anti-doping field. In this edition of the annual banned-substance review, literature on recent developments in anti-doping published between October 2020 and September 2021 is summarized and discussed, particularly focusing on human doping controls and potential applications of new testing strategies to substances and methods of doping specified in the World Anti-Doping Agency's 2021 Prohibited List. 

Original document


17 November 2021
Geyer, Hans
Kuuranne, Tiia
Thevis, Mario
Legal Terms
WADA Code, Guidelines, Protocols, Rules & Regulations
WADA Prohibited List International Standard
Other organisations
Centre Hospitalier Universitaire Vaudois (CHUV) - Lausanne University Hospital
Deutsche Sporthochschule Köln (DSHS) - German Sport University Cologne
European Monitoring Center for Emerging Doping Agents (EuMoCEDA)
Cologne, Germany: Institute of Biochemistry - German Sport University Cologne
Lausanne, Switzerland: Laboratoire Suisse d’Analyse du Dopage
Analytical aspects
Mass spectrometry analysis
Doping classes
M1. Manipulation Of Blood And Blood Components
M2. Chemical And Physical Manipulation
M3. Gene And Cell Doping
P1. Beta-Blockers
S0. Non-Approved Substances
S1. Anabolic Agents
S2. Peptide Hormones, Growth Factors
S3. Beta-2 Agonists
S4. Hormone And Metabolic Modulators
S5. Diuretics and Other Masking Agents
S6. Stimulants
S7. Narcotics
S8. Cannabinoids
S9. Glucocorticosteroids
Medical terms
Substance use research
Document category
Scientific article
Document type
Pdf file
Date generated
1 December 2021
Date of last modification
1 February 2022
  • Legal Source
  • Education
  • Science
  • Statistics
  • History
Country & language
  • Country
  • Language
Other filters
  • ADRV
  • Legal Terms
  • Sport/IFs
  • Other organisations
  • Laboratories
  • Analytical aspects
  • Doping classes
  • Substances
  • Medical terms
  • Various
  • Version
  • Document category
  • Document type
Publication period