Testing for natural and synthetic anabolic agents in human urine / C. Ayotte, D. Goudreault, A. Charlebois. – (Journal of Chromatography B: Biomedical Applications (1996) 687 (6 December) : p. 3-25)
- PMID: 9001949
This paper describes a comprehensive method for the detection of natural and synthetic anabolic agents, including some veterinary preparations such as trenbolone, zeranol (a non-steroidal agent) and clenbuterol (a beta 2-agonist). For the natural steroids such as testosterone, the precise determination of urinary androgens during routine procedures allowed the description of statistical distribution of relevant parameters of the endogenous steroid profile amongst male athletes. The validity of the results is discussed, taking into account some factors that may cause the degradation of the specimen.
Content:
1.) Introduction
2.) Experimental
2.1.) Standards
2.2.) Chemicals, solvents, reagents
2.3.) Enzymatic preparations
2.4.) Trimethylsilyl derivatizations
2.5.) Preparation of calibration standards
2.5.1.) Testoserone and epitestosterone
2.5.2.) Androsterone and etiocholanolone
2.6.) Isolation of the free and glucuroconjugated substances
2.7.) Isolation of the sulfoconjugated substances
2.8.) GC and GC-MS
2.9.) Luteinizing hormone (LH) measurements
3.) Results and discussion
3.1.) GC-MS detection and identification of anabolic agents
3.2.) Detection of testosterone administration and determination of the steroid profile
3.3.) Degradation of urinary samples
4.) Conclusion
