Steroidomics for highlighting novel serum biomarkers of testosterone doping

Steroidomics for highlighting novel serum biomarkers of testosterone doping / Federico Ponzetto, Julien Boccard, Raul Nicoli, Tiia Kuuranne, Martial Saugy, Serge Rudaz. - (Bioanalysis 11 (2019) 12 (10 June); p. 1171-1187).
- PMID: 31179715.
- DOI: 10.4155/bio-2019-0079


Abstract:

Aim: Quantification of testosterone (T) and 5α-dihydrotestosterone serum concentrations proved to be an efficient alternative to urinary steroid profiling for the detection of T doping. In this context, additional serum markers could be discovered by exploratory untargeted steroidomics studies.

Results: Endogenous steroid metabolites were monitored by ultra high-performance liquid chromatography coupled to high-resolution mass spectrometry in serum samples collected during a T administration clinical trial. A three-step workflow for accurate review of annotation was used and multifactorial data analysis allowed highlighting promising serum biomarkers. Longitudinal monitoring of selected compounds was performed to assess T abuse detection capabilities.

Conclusion: Application of serum steroidomics showed high potential for biomarker discovery of T doping, suggesting longitudinal monitoring of steroid hormones in serum as a significant improvement in detection of endogenous steroids abuse.

Parameters

Science
Study
Date
10 June 2019
People
Boccard, Julien
Kuuranne, Tiia
Nicoli, Raul
Ponzetto, Federico
Rudaz, Serge
Saugy, Martial
Country
Switzerland
Language
English
Other organisations
Lausanne Laboratory for doping analysis
Universität Basel - University of Basel
Université de Genève - University of Geneva
University of Lausanne
Laboratories
Lausanne, Switzerland: Laboratoire Suisse d’Analyse du Dopage
Analytical aspects
Testing method development
Doping classes
S1. Anabolic Agents
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Abstract
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9 September 2019
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11 September 2019
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