Detection of Black Market Follistatin 344

Detection of Black Market Follistatin 344 / Christian Reichel, Günter Gmeiner, Mario Thevis. - (Drug Testing and Analysis (2019) 23 November).
- PMID: 31758732.
- DOI: 10.1002/dta.2741


Abstract

Follistatin, a myostatin-inhibiting protein, is prohibited according to chapter S4 of the "WADA 2019 List of Prohibited Substances and Methods". While currently no approved pharmaceutical formulations of follistatin are available, follistatin can be bought on the black market. Most of the products are labelled "follistain 344" (FS344), few "follistain 315". A study on FS344 black market products was performed and an electrophoretic detection method for serum and urine developed. While only 9 of the 17 tested products actually contained follistatin, in some of the others growth promoting peptides were found (e.g. MGF, GHRP-2). Surprisingly, all nine products contained His-tagged FS344 and a high degree of its oligomers. The detection method is based on immunomagnetic purification followed by SDS-PAGE and Westen blotting with a monoclonal anti-His antibody. Alternatively, a monoclonal anti-follistatin antibody can be used. For immunoprecipitation (IP), a polyclonal anti-follistatin antibody is applied. An evaluation of suitable antibodies for IP and immunoblotting is also presented. Furthermore, practically all currently available follistatin standards were investigated. The detection limit of the method for black market FS344 in urine is ca 0.1 ng/mL for 10 mL. For a sample volume of 100 μL, an LOD of 5 ng/mL could be achieved for serum. Due to the presence of His-tags an unambiguous differentiation from endogenous follistatin is possible.

Parameters

Science
Study
Date
23 November 2019
People
Gmeiner, Günter
Reichel, Christian
Thevis, Mario
Country
Austria
Germany
Language
English
Other organisations
Deutsche Sporthochschule Köln (DSHS) - German Sport University Cologne
European Monitoring Center for Emerging Doping Agents (EuMoCEDA)
Laboratories
Cologne, Germany: Institute of Biochemistry - German Sport University Cologne
Seibersdorf, Austria: Seibersdorf Labor GmbH Doping Control Laboratory
Doping classes
S4. Hormone And Metabolic Modulators
Substances
Follistatin
Various
Illegal production / trade
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Date generated
19 November 2019
Date of last modification
4 December 2019
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