A novel approach to the quantification of urinary aryl-propionamide-derived SARMs by UHPLC-MS/MS

A novel approach to the quantification of urinary aryl-propionamide-derived SARMs by UHPLC-MS/MS / Azamat Temerdashev, Ekaterina Dmitrieva, Alice Azaryan, Elina Gashimova. - (Biomedical Chromatography 34 (2020) 1 (January) ; p. 1-9)

  • PMID: 31734960
  • DOI: 10.1002/bmc.4700


A simple and sensitive procedure for the quantification of two commonly abused aryl‐propionamide‐derived selective androgen receptor modulators (SARMs), namely S‐4 (GTx‐007, andarine) and S‐22 (GTx‐024, MK‐2866, ostarine, enobosarm), has been described. Urine samples were prepared for analysis by means of a dispersive liquid–liquid microextraction using methanol and chloroform as dispersive and extracting solvents, respectively. Factors that might influence the extraction process as well as their optimum conditions were evaluated by Box–Benken and central composite designs. After extraction, the analytes were quantified by UHPLC–MS/MS. The proposed procedure was validated on human urine samples. As a result, for both SARMs the detection limits were observed at 0.05 ng/mL and calibration curves were linear in the concentration range of 0.25–50 ng/mL with the coefficient of determination of 0.998.


Research / Study
17 November 2019
Azaryan, Alice
Dmitrieva, Ekaterina
Gashimova, Elina
Temerdashev, Azamat
Russian Federation
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Кубанский государственный университет (KubSU) - Kuban State University
Analytical aspects
Mass spectrometry analysis
Testing method development
Doping classes
S1. Anabolic Agents
Enobosarm (ostarine)
Selective androgen receptor modulators (SARMs)
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Date generated
14 January 2020
Date of last modification
7 September 2020
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