The detection of trans gene fragments of hEPO in gene doping model mice by Taqman qPCR assay

The detection of trans gene fragments of hEPO in gene doping model mice by Taqman qPCR assay / Kai Aoki, Takehito Sugasawa, Kouki Yanazawa, Koichi Watanabe, Tohru Takemasa, Yoshinori Takeuchi, Yuichi Aita, Naoya Yahagi, Yasuko Yoshida, Tomoaki Kuji, Nanami Sekine, Kaoru Takeuchi, Haruna Ueda, Yasushi Kawakami, Kazuhiro Takekoshi. - (PeerJ 8 (2020) e8595 (25 February); p. 1-14).
- doi: 10.7717/peerj.8595.
- PMCID: PMC7047860.
- PMID: 32140302
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Abstract

Background
With the rapid progress of genetic engineering and gene therapy methods, the World Anti-Doping Agency has raised concerns regarding gene doping, which is prohibited in sports. However, there is no standard method available for detecting transgenes delivered by injection of naked plasmids. Here, we developed a detection method for detecting transgenes delivered by injection of naked plasmids in a mouse model that mimics gene doping.

Methods
Whole blood from the tail tip and one piece of stool were used as pre-samples of injection. Next, a plasmid vector containing the human erythropoietin (hEPO) gene was injected into mice through intravenous (IV), intraperitoneal (IP), or local muscular (IM) injection. At 1, 2, 3, 6, 12, 24, and 48 h after injection, approximately 50 µL whole blood was collected from the tail tip. One piece of stool was collected at 6, 12, 24, and 48 h. From each sample, total DNA was extracted and transgene fragments were analyzed by Taqman quantitative PCR (qPCR) and SYBR green qPCR.

Results
In whole blood DNA samples evaluated by Taqman qPCR, the transgene fragments were detected at all time points in the IP sample and at 1, 2, 3, 6, and 12 h in the IV and IM samples. In the stool-DNA samples, the transgene fragments were detected at 6, 12, 24, and 48 h in the IV and IM samples by Taqman qPCR. In the analysis by SYBR green qPCR, the transgene fragments were detected at some time point in both specimens; however, many non-specific amplicons were detected.

Conclusions
These results indicate that transgene fragments evaluated after each injection method of naked plasmids were detected in whole-blood and stool DNA samples. These findings may facilitate the development of methods for detecting gene doping.

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Science
Study
Date:
25 February 2020
People
Aita, Yuichi
Aoki, Kai
Kawakami, Yasushi
Kuji, Tamoaki
Sekine, Nanami
Sugasawa, Takehito
Takekoshi, Kazuhiro
Takemasa, Tohru
Takeuchi, Kaoru
Takeuchi, Yoshinori
Ueda, Haruna
Watanabe, Koichi
Yahagi, Naoya
Yanazawa, Kouki
Yasuko, Yoshida
Country
Japan
Language
English
Other organisations
Tsukuba International University
University of Tsukuba
Analytical aspects
Testing method development
Doping classes
M3. Gene And Cell Doping
S2. Peptide Hormones, Growth Factors
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Erythropoietin (EPO)
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Gene Therapy
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Scientific article
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  • ADRV
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