Quantification of Endogenous Steroid Sulfates and Glucuronides in Human Urine After Intramuscular Administration of Testosterone Esters

Quantification of Endogenous Steroid Sulfates and Glucuronides in Human Urine After Intramuscular Administration of Testosterone Esters / Guro Forsdahl, Katharina Zanitzer, Damir Erceg, Günter Gmeiner. - (Steroids 157 (2020) 108614 (May); p. 1-11).
- PMID: 32097612.
- DOI: 10.1016/j.steroids.2020.108614


For an effective detection of doping with pseudo-endogenous anabolic steroids, the urinary steroid profile is of high value. In this work, the aim was to investigate steroid metabolism disruption after exogenous intramuscular administration of different testosterone esters. The investigation focused on both sulfo - and glucoro conjugated androgens. A single intramuscular injection of either 1000 mg testosterone undecanoate (Nebido®) or a mixture of 30 mg testosterone propionate, 60 mg testosterone phenylpropionate, 60 mg testosterone isocaproate, and 100 mg testosterone decanoate (Sustanone®), was given to six healthy volunteers. Urine was collected throughout a testing period of 60 days. A LC-MS method was developed and validated for the analysis of eight conjugated steroids in their intact form. The results show that urinary changes in both sulfo - and glucuro conjugated steroid levels are prominent after the injection of testosterone esters. A promising potential marker for the intake of exogenous testosterone is the combined ratio of epitestosterone sulfate/epitestosterone glucuronide to testosterone sulfate/testosterone glucuronide ((ES/EG)/(TS/TG)) as a complementary biomarker for testosterone abuse. This represents a new piece of evidence to detect testosterone doping, representing a new approach and being independent from the metabolic connections of the markers in the steroid passport.

Original document


Research / Study
22 February 2020
Erceg, Damir
Forsdahl, Guro
Gmeiner, Günter
Zanitzer, Katharina
Adverse Analytical Finding / presence
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Children's hospital Srebrnjak
Hrvatsko katoličko sveučilište - Catholic University of Croatia
Sv. Katarina Specijalna bolnica - St. Catherine Specialty Hospital
Sveučilište Josipa Jurja Strossmayera u Osijeku (UNIOS) - Josip Juraj Strossmayer University of Osijek
Universitetet i Tromsø – Norges arktiske universitet - University of Tromsø - The Arctic University of Norway
Seibersdorf, Austria: Seibersdorf Labor GmbH Doping Control Laboratory
Analytical aspects
Testing method development
Doping classes
S1. Anabolic Agents
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Date generated
30 April 2020
Date of last modification
5 May 2020
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