Alternative markers for Methylnortestosterone misuse in human urine

Alternative markers for Methylnortestosterone misuse in human urine / Panagiotis Sakellariou, Polyxeni Kiousi, Argyro G. Fragkaki, Emmanouil Lyris, Michael Petrou, Costas Georgakopoulos, Yiannis S. Angelis. - (Drug Testing and Analysis (2020, 30 June))

  • PMID: 32602999
  • DOI: 10.1002/dta.2887


Abstract

Methylnortestosterone is a progestin and synthetic androgenic anabolic steroid, prohibited by WADA. Methylnortestosterone misuse is commonly detected by monitoring the parent compound and its main metabolites, 17α-methyl-5α-estrane-3α, 17β-diol (M1) and 17α-methyl-5β-estrane-3α, 17β-diol (M2), in the glucuronide fraction. In the current study, a direct detection of methylnortestosterone sulfo-conjugated metabolites after ethyl acetate extraction and analysis by LC/Q/TOF-MS in negative ionization mode was performed, detecting two main sulfate metabolites (S1, S2). For the characterization of metabolites, samples from the excretion study, were additionally analyzed by GC-MS, after solvolysis and per TMS derivatization. RT and MS data collected, were compared with RT and MS data from metabolites of 17z-methyl-5α/β-estrane-3α/β, 17z-diols structures with prefixed stereochemistry at 3 and 5 positions, synthesized through Grignard reaction from 19-noretiocholanolone, 19-norandrosterone and 19-norepiandrosterone. Confirmed sulfate metabolites were S1, 17α-methyl-5α-estrane-3α, 17β-diol 3α sulfate (detected up to 72 h) and S2, 17α-methyl-5β-estrane-3α, 17β-diol 3α sulfate (detected up to 192 h). Furthermore, applying targeted analysis based on RT and MS data of the synthesized metabolites two additional metabolites M3, 17β-methyl-5β-estrane-3α, 17α-diol and M4, 17β-methyl-5α-estrane-3α, 17α-diol were detected in the glucuronide fraction and one more metabolite (S3) 17β-methyl-5β-estrane-3α, 17α-diol was detected in the sulfate fraction in lower abundance until the end of the excretion study (192 h). Interestingly, S2 could also be detected after the direct analysis of non-hydrolyzed steroid by GC-MS/MS as artifact, following normal ProcIV anabolic steroid procedure and using diethylether as extraction solvent.

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Science
Study
Date
30 June 2020
People
Angelis, Yanis S.
Fragkaki, Argyro G.
Georgakopoulos, Costas
Kiousi, Polyxeni
Lyris, Emmanouil
Petrou, Michael
Sakellariou, Panagiotis
Country
Cyprus
Greece
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Language
English
Other organisations
Cyprus Anti-Doping Authority (CyADA)
Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών - National and Kapodistrian University of Athens (NKUA)
Laboratories
Athens, Greece: Doping Control Laboratory of Athens
Doha, Qatar: Antidoping Lab Qatar, Doping Analysis Lab
Analytical aspects
Mass spectrometry analysis
Testing method development
Doping classes
S1. Anabolic Agents
Substances
Methyltestosterone
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Date generated
15 October 2020
Date of last modification
26 October 2020
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