Searching for new long-term urinary metabolites of metenolone and drostanolone using gas chromatography-mass spectrometry with a focus on non-hydrolysed sulfates

Searching for new long-term urinary metabolites of metenolone and drostanolone using gas chromatography-mass spectrometry with a focus on non-hydrolysed sulfates / Aðalheiður Dóra Albertsdóttir, Wim Van Gansbeke, Gilles Coppieters, Kyzylkul Balgimbekova, Peter Van Eenoo, Michael Polet. - (Drug Testing and Analysis 12 (2020) 8 (August); p. 1041-1053)

  • PMID: 32386339
  • DOI: 10.1002/dta.2818

  Abstract

Sulfated metabolites have been shown to have potential as long‐term markers of anabolic–androgenic steroid (AAS) abuse. In 2019, the compatibility of gas chromatography–mass spectrometry (GC–MS) with non‐hydrolysed sulfated steroids was demonstrated, and this approach allowed the incorporation of these compounds in a broad GC–MS initial testing procedure at a later stage. However, research is needed to identify which are beneficial.

In this study, a search for new long‐term metabolites of two popular AAS, metenolone and drostanolone, was undertaken through two excretion studies each. The excretion samples were analysed using GC–chemical ionization–triple quadrupole MS (GC–CI–MS/MS) after the application of three separate sample preparation methodologies (i.e. hydrolysis with Escherichia coli–derived β‐glucuronidase, Helix pomatia–derived β‐glucuronidase/arylsulfatase and non‐hydrolysed sulfated steroids).

For metenolone, a non‐hydrolysed sulfated metabolite, 1β‐methyl‐5α‐androstan‐17‐one‐3ζ‐sulfate, was documented for the first time to provide the longest detection time of up to 17 days. This metabolite increased the detection time by nearly a factor of 2 in comparison with the currently monitored markers for metenolone in a routine doping control initial testing procedure. In the second excretion study, it prolonged the detection window by 25%.

In the case of drostanolone, the non‐hydrolysed sulfated metabolite with the longest detection time was the sulfated analogue of the main drostanolone metabolite (3α‐hydroxy‐2α‐methyl‐5α‐androstan‐17‐one) with a detection time of up to 24 days. However, the currently monitored main drostanolone metabolite in routine doping control, after hydrolysis of the glucuronide with E.coli, remained superior in detection time (i.e. up to 29 days).

Parameters

Science
Study
Date
9 May 2020
People
Albertsdóttir, Aðalheiður Dóra Albertsdóttir
Coppieters, Gilles
Polet, Michael
Van Eenoo, Peter
Van Gansbeke, Wim
Country
Belgium
Kazakhstan
Language
English
Laboratories
Almaty, Kazakhstan: Athletes’ Anti-Doping Laboratory [*]
Ghent, Belgium: DoCoLab Universiteit Gent-UGent
Analytical aspects
Mass spectrometry analysis
Testing method development
Doping classes
S1. Anabolic Agents
Substances
Drostanolone
Metenolone
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5 November 2020
Date of last modification
6 November 2020
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