Investigation of stability of selective androgen receptor modulators in urine

Investigation of stability of selective androgen receptor modulators in urine /  Emiliano Ventura, Anna Gadaj, Tom Buckley, Mark H. Mooney. - (Food Additives & Contaminats: Part A 37 (2020) 8 (August); p. 1253-1263)

  • PMID: 32401172
  • DOI: 10.1080/19440049.2020.1757165


Abstract

Selective androgen receptor modulators (SARMs) are a class of new emerging "designer" steroid compounds gaining popularity over more well established anabolic-androgenic steroids (AAS) amongst both non-professional and elite athletes. Moreover, due to their anabolic activity, SARM compounds may also potentially be abused in livestock animals to increase meat production. Consequently, SARM residues should be monitored as a part of routine testing employed within both anti-doping and drug residue laboratories. Since only a limited amount of information on SARM compound stability is currently available within the peer-reviewed literature, this study reports a practical approach to assess optimal storage conditions for 15 SARM compounds in solvent solutions (standard stock and intermediate mixed standard solutions) stored at -20°C for up to 1 year, as well as in a range of urine test matrices (bovine, equine, canine and human) under frozen (-20°C, -80°C) storage for up to 20 weeks and post freeze-thaw cycles. Moreover, SARM storage stability within solvent extracts was assessed at -20°C (0-4 weeks) and 4°C (0-2 weeks). Findings demonstrate that SARM analytes are stable in reference solutions when stored at -20°C, apart from PF-06260414 (stock solution) which should be stored at lower temperatures (e.g. -80°C). A limited degree of compound instability was observed for a number of SARM analytes in urine both when stored at -20°C, and after repeated freeze-thaw cycles. Moreover, SARM compounds within reconstituted urine solvent extracts were found to be effectively stable when stored for up to 4 weeks at -20°C and for 2 weeks at 4°C. The long-term stability testing data reported here will inform the more timely and effective development and validation of analytical methods for SARM residue detection and analysis, ensuring confidence in findings from monitoring of livestock animals and anti-doping processes.

Original document

Parameters

Science
Study
Date
13 May 2020
People
Buckley, Tom
Gadaj, Anna
Mooney, Mark H.
Ventura, Emiliano
Country
Ireland
United Kingdom
Language
English
Other organisations
Irish Diagnostic Laboratory Services Ltd
Queen's University Belfast (QUB)
Analytical aspects
Mass spectrometry analysis
Doping classes
S1. Anabolic Agents
Substances
Selective androgen receptor modulators (SARMs)
Various
Contamination
Food and/or drinks
Meat contamination
Document category
Scientific article
Document type
Pdf file
Date generated
28 January 2021
Date of last modification
3 February 2021
Category
  • Legal Source
  • Education
  • Science
  • Statistics
  • History
Country & language
  • Country
  • Language
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  • ADRV
  • Legal Terms
  • Sport/IFs
  • Other organisations
  • Laboratories
  • Analytical aspects
  • Doping classes
  • Substances
  • Medical terms
  • Various
  • Version
  • Document category
  • Document type
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Origin