Survey of protein-based sport supplements for illegally added anabolic steroids methyltestosterone and 4-androstenedione by UPLC-MS/MS

Survey of protein-based sport supplements for illegally added anabolic steroids methyltestosterone and 4-androstenedione by UPLC-MS/MS / Somaye Alaedini, Maryam Amirahmadi, Farzad Kobarfard, Hossein Rastegar, Sasan Nasirahmadi, Shahram Shoeibi. - (Steroids 165 (2021) 108758 (January))

  • PMID: 33161054
  • DOI: 10.1016/j.steroids.2020.108758


Abstract

There is some evidence that marketable supplements contain hormones not declared on the product label. The presence of these androgenic anabolic steroids (AAS) in sports supplements can be considered an adulteration and affect the health of consumers, who are predominantly athletes. This study aimed to measure anabolic hormones (methyltestosterone and 4-androstenedione) in sport supplements. Ultra Performance Liquid chromatography coupled mass spectrometry (UPLC-MS/MS) with electrospray ionization (ESI) in positive mode was employed under the Multiple Reaction Monitoring (MRM) ion program. To overcome matrix effects and quantify the selected analyte, the calibration curve was made using Matrix Match method. The LOQ and LOD were 1 ng/g and 0.3 ng/g for both analytes. The recovery of 4-androstenedione and methyltestosterone was in the range of 86.87-107.35 and 77.31-113.98, respectively. In terms of reproducibility, CV % for 4-androstenedione and methyltestosterone ranged from 6.56 to 16.87% and 1.45-15.12%, respectively. 4-androstenedione was found in 11 samples including 9 whey as 1.578 ± 0.154 ng/g and 2 whey albumin samples with an amount of 1.134 ng/g and 1.474 ng/g. Consequently, continuous controlling of sport supplements comprising intentionally or unintentionally added androgens could be important for health and discuss in the context of compliance with anti-doping.

Parameters

Science
Study
Date
5 November 2020
People
Alaedini, Somaye
Amirahmadi, Maryam
Kobarfard, Farzad
Nasirahmadi, Sasan
Rastegar, Hossein
Shoeibi, Shahram
Country
Iran, Islamic Republic of
Language
English
Other organisations
Iranian Ministry of Health and Medical Education (MOHME)
Islamic Azad University (IAU)
Analytical aspects
Mass spectrometry analysis
Testing method development
Doping classes
S1. Anabolic Agents
Substances
4-Androstenediol (androst-4-ene-3β,17β-diol)
Various
Contamination
Supplements
Document category
Abstract
Date generated
22 February 2021
Date of last modification
3 March 2021
Category
  • Legal Source
  • Education
  • Science
  • Statistics
  • History
Country & language
  • Country
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  • ADRV
  • Legal Terms
  • Sport/IFs
  • Other organisations
  • Laboratories
  • Analytical aspects
  • Doping classes
  • Substances
  • Medical terms
  • Various
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  • Document category
  • Document type
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Origin