Analysis of anabolic steroids using GC/MS with selected ion monitoring

Analysis of anabolic steroids using GC/MS with selected ion monitoring / Bong Chul Chung, Hea-Young P. Choo, Tae Wook Kim, Khee Dong Eom, Oh Seung Kwon, Jawon Suh, Jongsoon Yang, Jongsei Park. - (Journal of Analytical Toxicology 14 (1990) 2 (March-April); p. 91-95)

  • PMID: 2325383
  • DOI: 10.1093/jat/14.2.91


Abstract

This study describes the use of gas chromatography/mass spectrometry with selected ion monitoring to screen 18 anabolic steroids banned by the International Olympic Committee. These anabolic steroids are analyzed in two fractions depending on their excretion pattern: nonconjugated (free) or conjugated fraction. The wet procedure of extracting steroids from urine consists of an initial isolation of lipophilic compounds on a column packed with Amberlite XAD-2 resin, followed by enzymatic hydrolysis with beta-glucuronidase from Escherichia coli. After extraction, the hydrolyzed steroids are derivatized to the corresponding trimethylsilyl ethers. The derivatized steroids are analyzed by gas chromatography/mass spectrometry with selected ion monitoring of their characteristic ions. It takes 12 and 26 min to run GC/MS and edit the raw data for nonconjugated and conjugated fractions respectively.

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Science
Study
Date
1 March 1990
People
Choo, Hea-Young P.
Chung, Bong Chul
Eom, Khee Dong
Kim, Tae Wook
Kwom, Oh Seung
Park, Jongsei
Suh, Jawon
Yang, Jonsoon
Country
Korea, Republic of
Language
English
Laboratories
Seoul, Korea: Doping Control Center
Analytical aspects
Testing method development
Doping classes
S1. Anabolic Agents
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Scientific article
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3 March 2021
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