Analysis of anabolic steroids using GC/MS with selected ion monitoring / Bong Chul Chung, Hea-Young P. Choo, Tae Wook Kim, Khee Dong Eom, Oh Seung Kwon, Jawon Suh, Jongsoon Yang, Jongsei Park. - (Journal of Analytical Toxicology 14 (1990) 2 (March-April); p. 91-95)
- PMID: 2325383
- DOI: 10.1093/jat/14.2.91
Abstract
This study describes the use of gas chromatography/mass spectrometry with selected ion monitoring to screen 18 anabolic steroids banned by the International Olympic Committee. These anabolic steroids are analyzed in two fractions depending on their excretion pattern: nonconjugated (free) or conjugated fraction. The wet procedure of extracting steroids from urine consists of an initial isolation of lipophilic compounds on a column packed with Amberlite XAD-2 resin, followed by enzymatic hydrolysis with beta-glucuronidase from Escherichia coli. After extraction, the hydrolyzed steroids are derivatized to the corresponding trimethylsilyl ethers. The derivatized steroids are analyzed by gas chromatography/mass spectrometry with selected ion monitoring of their characteristic ions. It takes 12 and 26 min to run GC/MS and edit the raw data for nonconjugated and conjugated fractions respectively.
