Relevance of the selective oestrogen receptor modulators tamoxifen, toremifene and clomiphene in doping field: endogenous steroids urinary profile after multiple oral doses

Relevance of the selective oestrogen receptor modulators tamoxifen, toremifene and clomiphene in doping field : endogenous steroids urinary profile after multiple oral doses / Monica Mazzarino, Maria Cristina Braganò, Xavier de la Torre, Francesco Molaioni, Francesco Botrè. - (Steroids 76 (2011) 12 (November); p. 1400-1406)

  • PMID: 21745489
  • DOI: 10.1016/j.steroids.2011.06.005


Abstract

The present study was performed to investigate the influence of the intake of selective oestrogen receptor modulators on the urinary endogenous steroids profile. For this purpose the circadian variability of luteinizing hormone, follicle-stimulating hormone, testosterone, 5α-androstan-3α,17β-diol, 5β-androstan-3α,17β-diol, epitestosterone, 4-androstenedione, androsterone and etiocholanolone were measured on eight subjects (four males and four females) by gas chromatography-mass spectrometry and chemiluminescent immunometric assay techniques before and after oral administration of multiple doses of either tamoxifen (80 mg for 2 days) or toremifene (120 mg for 2 days) or clomiphene (100 mg for 2 days). The individual baseline variability of the steroids studied was set up by collecting the urine samples every 3 h, for 3 days prior to the treatment; whereas the evaluation of the effects of the oral administration of multiple doses of selective oestrogen receptor modulators on the steroid urinary profile was assessed by collecting urine samples every three hours for at least five days from the first administration.

The results of our measurements showed that, only in male subjects, the relative urinary concentrations of testosterone, epitestosterone and 4-androstenedione were significantly altered generally after the second day of drug administration. While no significant effects were recorded in both sexes on the luteinizing hormone, follicle-stimulating hormone, androsterone, etiocholanolone, 5α-androstan-3α,17β-diol and 5β-androstan-3α,17β-diol urinary levels and on testosterone/epitestosterone, 5α-androstan-3α,17β-diol/5β-androstan-3α,17β-diol and androsterone/etiocholanolone ratios.

Original document

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Science
Study
Date
30 June 2011
People
Botrè, Francesco
Braganò, Maria Cristina
de la Torre, Xavier
Mazzarino, Monica
Molaioni, Francesco
Country
Italy
Language
English
Other organisations
Sapienza Università di Roma - Sapienza University of Rome
Laboratories
Roma, Italia: Laboratorio Antidoping FMSI
Analytical aspects
Mass spectrometry analysis
Testing method development
Doping classes
S4. Hormone And Metabolic Modulators
Substances
Clomifene
Selective estrogen receptor modulators (SERMS)
Tamoxifen
Toremifene
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Scientific article
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9 March 2021
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12 March 2021
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