Elevated urinary cobalt concentrations identified in routine doping controls can originate from vitamin B12

Elevated urinary cobalt concentrations identified in routine doping controls can originate from vitamin B12 / Andre Knoop, Christian Görgens, Hans Geyer, Mario Thevis. - (Rapid Communications in Mass Spectrometry 34 (2019) 7 (15 April); p. 1-3)

  • DOI: 10.1002/rcm.8649

Cyanocobalamin was unequivocally identified in the doping control urine sample by HRMS. The intact molecular ion was observed in full‐MS measurements, and diagnostic product ions obtained by collision‐induced dissociation were detected and matched previous publications.21 It is noteworthy that a concentration of 565 ng/mL was calculated using the prepared calibration curve, computed by signal area ratios of the target analyte and the employed internal standard (fivefold deuterated isoxsuprine, ISTD).

The presented data indicate the importance of the careful assessment of total urinary cobalt concentrations in the context of routine sports drug testing programs and, potentially, also in other clinical applications. The determination of inorganic cobalt and the differentiation from cyanocobalamin can be accommodated by simple SPE‐based depletion of the cobalt‐containing organic molecule and subsequent analysis of the extract's flow‐through by ICP‐MS. Alternatively, routine tests by means of LC‐ICP‐MS and/or concomitant analyses of urine samples for vitamin B12 are recommended for adequate result interpretation and management.

Original document


Research / Study
12 November 2019
Geyer, Hans
Görgens, Christian
Knoop, Andre
Thevis, Mario
Other organisations
Deutsche Sporthochschule Köln (DSHS) - German Sport University Cologne
Cologne, Germany: Institute of Biochemistry - German Sport University Cologne
Analytical aspects
Mass spectrometry analysis
Testing method development
Doping classes
S2. Peptide Hormones, Growth Factors
Document category
Scientific article
Document type
Pdf file
Date generated
10 March 2021
Date of last modification
18 March 2021
  • Legal Source
  • Education
  • Science
  • Statistics
  • History
Country & language
  • Country
  • Language
Other filters
  • ADRV
  • Legal Terms
  • Sport/IFs
  • Other organisations
  • Laboratories
  • Analytical aspects
  • Doping classes
  • Substances
  • Medical terms
  • Various
  • Version
  • Document category
  • Document type
Publication period