Detection of formestane abuse by mass spectrometric techniques

Detection of formestane abuse by mass spectrometric techniques / Xavier de la Torre, Cristiana Colamonici, Davide Curcio, Daniel Jardines, Francesco Molaioni, Maria Kristina Parr, Francesco Botrè. - (Drug Testing and Analysis 6 (2014) 11-12 (November-December); p. 1133-1140)

  • PMID: 25516450
  • DOI: 10.1002/dta.1759

Abstract

Formestane (4-hydroxy-androstenedione) is an aromatase inhibitor prohibited in sports and included, since 2004, in the list of prohibited substances updated yearly by the World Anti-Doping Agency (WADA). Since the endogenous production of formestane has been described, it is mandatory for the anti-doping laboratories to use isotope ratio mass spectrometry (IRMS) to establish the exogenous origin before issuing an adverse analytical finding. The described IRMS methods for formestane detection are time-consuming, requiring usually two consecutive liquid chromatographic sample purifications in order to have final extracts of adequate purity before the mass spectrometric analysis. After establishing a procedure for the determination of the origin of formestane by IRMS without the need of derivatization, and integrated in the overall analytical strategy of the laboratory for pseudo-endogenous steroids, a mass spectrometric analysis by gas chromatography-mass spectrometry (GC-MS) and gas chromatography-tandem mass spectrometry (GC-MS/MS) of formestane metabolites was carried out in order to investigate whether other biomarkers of formestane abuse could be integrated in order to avoid time-consuming and expensive IRMS confirmations for formestane. From the metabolic studies performed, the inclusion of 3β,4α-dihydroxy-5α-androstan-17-one (4α-hydroxy-epiandosterone) in the routine GC-MS procedures has demonstrated to be diagnostic in order to reduce the number of unnecessary confirmations of the endogenous origin of formestane.

Original document

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Science
Study
Date
16 December 2014
People
Botrè, Francesco
Colamonici, Cristiana
Curcio, Davide
de la Torre, Xavier
Jardines, Daniel
Molaioni, Francesco
Parr, Maria Kristina
Country
Italy
Language
English
Other organisations
Sapienza Università di Roma - Sapienza University of Rome
Laboratories
Roma, Italia: Laboratorio Antidoping FMSI
Analytical aspects
Mass spectrometry analysis
Testing method development
Doping classes
S4. Hormone And Metabolic Modulators
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Formestane
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Scientific article
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15 March 2021
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18 March 2021
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