Analysis of conjugated steroid androgens: deconjugation, derivatisation and associated issues

Analysis of conjugated steroid androgens : deconjugation, derivatisation and associated issues / Rachel L. Gomes, Will Meredith, Colin E. Snape, Mark A. Sephton. - (Journal of Pharmaceutical and Biomedical Analysis 49 (2009) 5 (12 July); p. 1133-1140)

  • PMID: 19304432
  • PMCID: PMC2684592
  • DOI: 10.1016/j.jpba.2009.01.027


Gas chromatography/mass spectrometry (GC/MS) is the preferred technique for the detection of urinary steroid androgens for drug testing in athletics. Excreted in either the glucuronide or sulfated conjugated form, steroids must first undergo deconjugation followed by derivatisation to render them suitable for GC analysis. Discussed herein are the deconjugation and the derivatisation preparative options. The analytical challenges surrounding these preparatory approaches, in particular the inability to cleave the sulfate moiety have led to a focus on testing protocols that reply on glucuronide conjugates. Other approaches which alleviate the need for deconjugation and derivatisation are also highlighted.

Original document


31 January 2009
Gomes, Rachel L.
Meredith, Will
Sephton, Mark A.
Snape, Colin E.
United Kingdom
University of Nottingham
Other organisations
Imperial College London
Analytical aspects
Mass spectrometry analysis
Testing method development
Document type
Pdf file
Date generated
31 March 2021
Date of last modification
15 April 2021
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