Analytical strategy for the detection of ecdysterone and its metabolites in vivo in uPA(+/+)-SCID mice with humanised liver, human urine samples and estimation of prevalence of its use in anti-doping samples

Analytical strategy for the detection of ecdysterone and its metabolites in vivo in uPA(+/+)-SCID mice with humanised liver, human urine samples and estimation of prevalence of its use in anti-doping samples / S. Kraiem, M.Y. Al-Jaber, H. Al-Mohammed, A.S. Al-Menhali, N.J. AlThani, M. Helaleh, W. Samsam, S. Touil, A. Beotra, C. Georgakopoulas, S. Bouabdallah, V. Mohamed-Ali, M. Almaadheed, - (Drug Testing and Analysis (2021) 23 March)

  • PMID: 33759363
  • DOI: 10.1002/dta.3032


Abstract

Ecdysteroids are of interest as potential sport performance enhancers, due to their anabolic effects. The current study aimed to analyse levels of the most abundant ecdysteroid, ecdysterone (20-hydroxyecdysone, 20-OHE) in easily available dietary supplements, and, outline an analytical strategy for its detection, and that, of its metabolites, 1) following administration of pure 20-OHE to uPA(+/+)-SCID mice with humanised liver, 2) in a human volunteer after ingestion of two supplements, one with a relatively low, and the other a high, concentration of 20-OHE, and, 3) to estimate the prevalence of use of 20-OHE in elite athletes (n=1000). Of the 16 supplements tested, only five showed detectable levels of 20-OHE, with concentrations ranging from undetectable up to 2.3 mg per capsule. Urine of uPA(+/+)-SCID urine showed the presence of 20-OHE and its metabolite, 14 deoxy ecdysterone, within 24 hours (h) of ingestion. In humans, both the parent and the metabolite were detectable within 2 to 5 h of ingestion, with the metabolite being detectable for longer than the parent. After ingestion of a low dose supplement, the parent and metabolite were detectable for 70 h and 48 h, while following the higher dose it was 96 h and 48 h, respectively. Analysis of urines from athletes (n=1000) confirmed four positives for 20-OHE, suggesting a prevalence of use of 0.4%. Prevalence of its use by elite athletes was relatively low, however, this needs to be confirmed in other populations, and with other related ecdysteroids.

Parameters

Science
Study
Date
23 March 2021
People
Al-Jaber, M.Y.
Al-Menhali, A.S.
Al-Mohammed, H.
Almaadheed, M.
AlThani, N.J.
Beotra, A.
Bouabdallah, S.
Georgakopoulas, C.
Helaleh, M.
Kraiem, S.
Mohamed-Ali, Vidya
Samsam, Waseem
Touil, S.
Country
Qatar
Tunisia
United Kingdom
Language
English
Other organisations
University College London (UCL)
University of Carthage
Laboratories
Doha, Qatar: Antidoping Lab Qatar, Doping Analysis Lab
Analytical aspects
New substance, not on prohibited list
Testing method development
Doping classes
S1. Anabolic Agents
Substances
Ecdysterone
Document category
Abstract
Date generated
22 April 2021
Date of last modification
4 May 2021
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  • Legal Source
  • Education
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  • Statistics
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  • Country
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  • ADRV
  • Legal Terms
  • Sport/IFs
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  • Laboratories
  • Analytical aspects
  • Doping classes
  • Substances
  • Medical terms
  • Various
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