Metabolism study of boldenone in human urine by gas chromatography-tandem mass spectrometry

Metabolism study of boldenone in human urine by gas chromatography-tandem mass spectrometry / Xinchen Wu, Feng Gao, Wenxin Zhang, Jian Ni. - (Journal of Pharmaceutical and Biomedical Analysis 115 (2015) 10 November; p. 570-575)

  • PMID: 26319750
  • DOI: 10.1016/j.jpba.2015.08.014


Boldenone (BOLD), an anabolic steroid, is likely to be abused in livestock breeding and in sports. Although some of BOLD metabolites in human urine, such as 5β-adrost-1-en-17β-ol-3-one (BM1), have been detected, investigations on their excretion patterns for both genders are insufficient. Moreover, little research on 17α-BOLD glucuronide as a metabolite in human urine has been reported. The aim of this study is to make a contribution to the knowledge of 17β-BOLD metabolism in humans. Three male and three female volunteers were orally administrated with 30mg 17β-BOLD. Urine samples were collected and analyzed with gas chromatography-tandem mass spectrometry. The data proved that 17β-BOLD, BM1, and 17α-BOLD were excreted in urine in both free and glucuronic conjugated forms after administration of 17β-BOLD. For most subjects, the urinary concentrations of BM1 were higher than that of 17β-BOLD. 17α-BOLD was excreted in small amounts. 17α-BOLD, 17β-BOLD, and BM1 were present naturally in urine with low concentrations. Administration of 30mg 17β-BOLD could not influence the excretion profiles of urinary androsterone, etiocholanolone, and testosterone / epitestosterone ratio. There were no differences in BOLD metabolic patterns between man and woman.

Original document


18 August 2015
Gao, Feng
Ni, Jian
Wu, Xinchen
Other organisations
Beijing University of Chinese Medicine
Kunming Medical University
Analytical aspects
Half-life / excretion time of substance
Mass spectrometry analysis
Doping classes
S1. Anabolic Agents
Medical terms
Health effects
Meat contamination
Document category
Scientific article
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Pdf file
Date generated
7 June 2021
Date of last modification
10 June 2021
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