Untargeted profiling of urinary steroid metabolites after testosterone ingestion: opening new perspectives for antidoping testing / Julien Boccard, Flavia Badoud, Nicolas Jan, Raul Nicoli, Carine Schweizer, François Pralong, Jean-Luc Veuthey, Norbert Baume, Serge Rudaz, Martial Saugy. - (Bioanalysis 6 (2014) 19; p. 2523-2536)
- PMID: 25411696
- DOI: 10.4155/bio.14.200
Aim: Antidoping procedures are expected to greatly benefit from untargeted metabolomic approaches through the discovery of new biomarkers of prohibited substances abuse.
Results: Endogenous steroid metabolites were monitored in urine samples from a controlled elimination study of testosterone undecanoate after ingestion. A platform coupling ultra-high pressure LC with high-resolution quadrupole TOF MS was used and high between-subject metabolic variability was successfully handled using a multiblock data analysis strategy. Links between specific subsets of metabolites and influential genetic polymorphisms of the UGT2B17 enzyme were highlighted.
Conclusion: This exploratory metabolomic strategy constitutes a first step toward a better understanding of the underlying patterns driving the high interindividual variability of steroid metabolism. Promising biomarkers were selected for further targeted study.