Affinity sensing for transgenes detection in antidoping control

Affinity sensing for transgenes detection in antidoping control / Simona Scarano, Maria M. Spiriti, Genny Tigli, Patrizia Bogani, Marcello Buiatti, Marco Mascini, Maria Minunn. - (Analytical Chemistry 81 (2009) 23 (1 December); p. 9571-9577)

  • PMID: 19877642
  • DOI: 10.1021/ac901445b


Sports authorities fear that a new form of doping called gene doping, based on the misuse of gene therapy, represents an emerging important problem and so far no methods are available for detecting it. The World Anti-Doping Agency (WADA) has included since 2003 for the first time gene doping methods in the "Prohibited List of Substances and Methods", thus detection of this new form of doping is challenging for analytical chemists. In this work, we apply affinity-based biosensors (ABBs), in particular DNA piezoelectric sensing, for detection of target DNA sequences selected as transgenosis markers. In this work, two sequences widely used in transgenosis experiments have been identified as markers: the enhanced green fluorescence protein (EGFP) gene and the promoter of Cytomegalovirus (CMV). The biosensors are characterized in their analytical performances using synthetic oligonucleotides and amplified DNA obtained from purified plasmid used as a template. Finally they have been applied to transgenic human cell cultures (human embryonic kidney HEK-EGFP), transformed with the same plasmid and carrying the target markers. This represents the closest human real matrix available for our transgenes.

Original document


Research / Study
30 October 2009
Bogani, Patrizia
Buiatti, Marcello
Minunn, Maria
Scarano, Simona
Spiriti, Maria M.
Tigli, Genny
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Università degli Studi di Firenze (UniFI) - University of Florence
Doping classes
M3. Gene And Cell Doping
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Scientific article
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Pdf file
Date generated
20 July 2021
Date of last modification
27 July 2021
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