Short term impact of Tribulus terrestris intake on doping control analysis of endogenous steroids

Short term impact of Tribulus terrestris intake on doping control analysis of endogenous steroids / Christophe Saudan, Norbert Baume, Caroline Emery, Emmanuel Strahm, Martial Saugy. - (Forensic Science International 178 (2008) 1 (10 June); p. e7-e10)

  • PMID: 18282674
  • DOI: 10.1016/j.forsciint.2008.01.003


Abstract

Tribulus terrestris is a nutritional supplement highly debated regarding its physiological and actual effects on the organism. The main claimed effect is an increase of testosterone anabolic and androgenic action through the activation of endogenous testosterone production. Even if this biological pathway is not entirely proven, T. terrestris is regularly used by athletes. Recently, the analysis of two female urine samples by GC/C/IRMS (gas chromatography/combustion/isotope-ratio-mass-spectrometry) conclusively revealed the administration of exogenous testosterone or its precursors, even if the testosterone glucuronide/epitestosterone glucuronide (T/E) ratio and steroid marker concentrations were below the cut-off values defined by World Anti-Doping Agency (WADA). To argue against this adverse analytical finding, the athletes recognized having used T. terrestris in their diet. In order to test this hypothesis, two female volunteers ingested 500 mg of T. terrestris, three times a day and for two consecutive days. All spot urines were collected during 48 h after the first intake. The (13)C/(12)C ratio of ketosteroids was determined by GC/C/IRMS, the T/E ratio and DHEA concentrations were measured by GC/MS and LH concentrations by radioimmunoassay. None of these parameters revealed a significant variation or increased above the WADA cut-off limits. Hence, the short-term treatment with T. terrestris showed no impact on the endogenous testosterone metabolism of the two subjects.

Original document

Parameters

Science
Research / Study
Date
20 March 2008
People
Baume, Norbert
Emery, Caroline
Saudan, Christophe
Saugy, Martial
Strahm, Emmanuel
Country
Switzerland
Language
English
ADRV
Adverse Analytical Finding / presence
Other organisations
Centre Hospitalier Universitaire Vaudois (CHUV) - Lausanne University Hospital
Université de Lausanne (UNIL) - University of Lausanne
Laboratories
Lausanne, Switzerland: Laboratoire Suisse d’Analyse du Dopage
Analytical aspects
Mass spectrometry analysis
New substance, not on prohibited list
Threshold for endogenous substances
Doping classes
S1. Anabolic Agents
Substances
T/E ratio (testosterone / epitestosterone)
Testosterone
Various
Doping control
Supplements
Document category
Scientific article
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Pdf file
Date generated
20 July 2021
Date of last modification
27 July 2021
Category
  • Legal Source
  • Education
  • Science
  • Statistics
  • History
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  • Country
  • Language
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  • ADRV
  • Legal Terms
  • Sport/IFs
  • Other organisations
  • Laboratories
  • Analytical aspects
  • Doping classes
  • Substances
  • Medical terms
  • Various
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  • Document category
  • Document type
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Origin