Preventive doping control analysis: liquid and gas chromatography time-of-flight mass spectrometry for detection of designer steroids

Preventive doping control analysis : liquid and gas chromatography time-of-flight mass spectrometry for detection of designer steroids / Costas G. Georgakopoulos, Ariadni Vonaparti, Marianna Stamou, Polyxeni Kiousi, Emmanouil Lyris, Yiannis S. Angelis, George Tsoupras, Bernhard Wuest, Michel W.F. Nielen, Irene Panderi, Michalis Koupparis. - (Rapid Communicaton in Mass Spectrometry 21 (2007) 15 (15 August); p. 2439-2446)

  • PMID: 17610244
  • DOI: 10.1002/rcm.3103


A new combined doping control screening method for the analysis of anabolic steroids in human urine using liquid chromatography/electrospray ionization orthogonal acceleration time-of-flight mass spectrometry (LCoaTOFMS) and gas chromatography/electron ionization orthogonal acceleration time-of-flight mass spectrometry (GCoaTOFMS) has been developed in order to acquire accurate full scan MS data to be used to detect designer steroids. The developed method allowed the detection of representative prohibited substances, in addition to steroids, at concentrations of 10 ng/mL for anabolic agents and metabolites, 30 ng/mL for corticosteroids, 500 ng/mL for stimulants and beta-blockers, 250 ng/mL for diuretics, and 200 ng/mL for narcotics. Sample preparation was based on liquid-liquid extraction of hydrolyzed human urine, and the final extract was analyzed as trimethylsilylated derivatives in GCoaTOFMS and underivatized in LCoaTOFMS in positive ion mode. The sensitivity, mass accuracy, advantages and limitations of the developed method are presented.

Original document


Research / Study
3 July 2007
Angelis, Yanis S.
Georgakopoulos, Costas G.
Kiousi, Polyxeni
Lyris, Emmanouil
Nielen, Michel W.F.
Panderi, Irene
Stamou, Marianna
Tsoupras, George
Vonaparti, Ariadni
Wuest, Bernhard
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Rijks- en Kwaliteitsinstituut voor Land- en Tuinbouwproducten (RIKILT)
Wageningen University & Research (WUR)
Εθνικό και Καποδιστριακό Πανεπιστήμιο Αθηνών - National and Kapodistrian University of Athens (NKUA)
Athens, Greece: Doping Control Laboratory of Athens
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Mass spectrometry analysis
Testing method development
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Scientific article
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Date generated
28 July 2021
Date of last modification
29 July 2021
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