A fast screening method for the detection of CERA in dried blood spots

A fast screening method for the detection of CERA in dried blood spots / Angela Rocca, Laurent Martin, Tiia Kuuranne, Magnus Ericsson, Alexandre Marchand, Nicolas Leuenberger. - (Drug Testing and Analysis (2021) 11 August); p. 1-6)

  • PMID: 34380180
  • DOI: 10.1002/dta.3142


Abstract

Continuous erythropoietin receptor activator (CERA) is a third-generation erythropoiesis-stimulating agent that was developed for the treatment of anemia. However, misuse of CERA for doping in endurance sports has been reported. Previous studies have shown blood as the matrix of choice for the detection of CERA, due to its high molecular weight. The use of dried blood spots (DBSs) for anti-doping purposes constitutes a complementary approach to the standard urine and venous blood matrices and could facilitate sample collection and increase the number of blood samples available for analysis due to reduced costs of sample collection and transport. Here, we investigated whether CERA could be indirectly detected in extracts of single DBSs using an erythropoietin-specific immunoassay that is capable of providing results within approximately 2 h. Reconstituted DBS samples were prepared from mixtures of red blood cell pellets and serum samples. The samples were collected in a previous clinical study in which six healthy volunteers were injected with a single, 200 μg dose of CERA. Using a commercially available ELISA kit, CERA was detected in the DBSs with a detection window of up to 20 days post-injection. Furthermore, in order to demonstrate the fitness-for-purpose, three authentic doping control serum samples, which were identified as containing CERA, were analyzed by the presented methodological approach on DBS. The testing procedure described here could be used as a fast and cost-effective method for the detection of CERA abuse in sport.

Original document

Parameters

Science
Study
Date
11 August 2021
People
Ericsson, Magnus
Kuuranne, Tiia
Leuenberger, Nicolas
Marchand, Alexandre
Martin, Laurent
Rocca, Angela
Country
France
Switzerland
Language
English
Laboratories
Lausanne, Switzerland: Laboratoire Suisse d’Analyse du Dopage
Paris, France: Agence Française de Lutte contre le Dopage (AFLD)
Analytical aspects
Blood testing method
Testing method development
Doping classes
S2. Peptide Hormones, Growth Factors
Substances
Methoxy polyethylene glycol-epoetin beta (CERA)
Document category
Scientific article
Document type
Pdf file
Date generated
30 August 2021
Date of last modification
1 September 2021
Category
  • Legal Source
  • Education
  • Science
  • Statistics
  • History
Country & language
  • Country
  • Language
Other filters
  • ADRV
  • Legal Terms
  • Sport/IFs
  • Other organisations
  • Laboratories
  • Analytical aspects
  • Doping classes
  • Substances
  • Medical terms
  • Various
  • Version
  • Document category
  • Document type
Publication period
Origin