Mass spectrometry-based characterization of new drugs and methods of performance manipulation in doping control analysis

Mass spectrometry-based characterization of new drugs and methods of performance manipulation in doping control analysis / Mario Thevis, Andreas Thomas, Maxie Kohler, Simon Beuck, Ines Möller, Mathias Schäfer, Grigory Rodchenkov, Sheng Yin, Joseph A. Loo, Hans Geyer, Wilhelm Schänzer. - (
European Journal of Mass Spectrometry 16 (2010) 3 (1 February); p. 301-312)

  • PMID: 20530837
  • DOI: 10.1255/ejms.1047


Efficient and comprehensive sports drug testing necessitates frequent updating and proactive, preventive anti-doping research, and the early implementation of new, emerging drugs into routine doping controls is an essential aspect. Several new drugs and drug candidates with potential for abuse, including so-called Rycals (ryanodine receptor calstabin complex stabilizers, for example, S-107), hypoxia-inducible factor (HIF) stabilizers, and peroxisome-proliferator-activated receptor (PPAR) delta agonists (for example, GW1516), were studied using different mass spectrometry- and ion mobility-based approaches, and their gas phase dissociation behaviors were elucidated. The detailed knowledge of fragmentation routes allows a more rapid identification of metabolites and structurally related, presumably "tailor-made", analogs potentially designed for doping purposes. The utility of product ion characterization is demonstrated in particular with GW1516, for which oxidation products were readily identified in urine samples by means of diagnostic fragment ions as measured using high resolution/high accuracy mass spectrometry and higher energy collision-induced dissociation (HCD).

Original document


Research / Study
1 February 2010
Beuck, Simon
Geyer, Hans
Kohler, Maxie
Loo, Joseph A.
Möller, Ines
Rodchenkov, Grigory
Schäfer, Mathias
Schänzer, Wilhelm
Thevis, Mario
Thomas, Andreas
Yin, Scheng
Russian Federation
Other organisations
Deutsche Sporthochschule Köln (DSHS) - German Sport University Cologne
Universität zu Köln - University of Cologne
University of California, Los Angeles (UCLA)
Moscow, Russia: Antidoping Centre Moscow [*]
Analytical aspects
Mass spectrometry analysis
Testing method development
Doping classes
S2. Peptide Hormones, Growth Factors
S4. Hormone And Metabolic Modulators
Hypoxia-inducible factor (HIF) activating agents
Peroxisome Proliferator Activated Receptor δ (PPARδ) agonists
Document category
Scientific article
Document type
Pdf file
Date generated
21 September 2021
Date of last modification
24 September 2021
  • Legal Source
  • Education
  • Science
  • Statistics
  • History
Country & language
  • Country
  • Language
Other filters
  • ADRV
  • Legal Terms
  • Sport/IFs
  • Other organisations
  • Laboratories
  • Analytical aspects
  • Doping classes
  • Substances
  • Medical terms
  • Various
  • Version
  • Document category
  • Document type
Publication period