Identification of the anabolic steroid 6β-chlorotestosterone in a dietary supplement / Travis M. Falconer, Rick A. Flurer, Mary B. Jones, Lisa M. Lorenz, Sarah E. Voelker
- Drug Testing and Analysis 11 (2019) 3 (March), p. 422-427
- PMID: 30238638
- DOI: 10.1002/dta.2510
Capsules that were labeled to be performance-enhancing dietary supplements obtained during an investigation were found to contain an unrecognized steroid-like substance. This compound was isolated by liquid chromatography (LC) fraction collection and characterized using several qualitative analytical techniques, including ultraviolet (UV) spectroscopy, gas chromatography-mass spectrometry (GC-MS), liquid chromatography-high resolution accurate mass-mass spectrometry (LC-HRAM-MS), as well as 1 H, 13 C, and two-dimensional nuclear magnetic resonance (NMR) spectrometry. This multi-technique analytical approach was used to identify the designer steroid as 6β-chloro-4-androsten-17β-ol-3-one (6β-chlorotestosterone), an analog of testosterone about which little has been published.