Annual banned-substance review: analytical approaches in human sports drug testing - [2019-2020]

Annual banned-substance review: analytical approaches in human sports drug testing / Mario Thevis, Tiia Kuuranne, Hans Geyer. - (Drug Testing and Analysis 13 (2021) 1 (January); p. 8-35)

  • PMID: 31724288
  • DOI: 10.1002/dta.2969


  • Introduction
  • Anabolic Agent
    • Anabolic-androgenic steroids
    • Initial testing procedures: Comprehensive screening, metabolism studies
    • Steroid profiling in urine and serum
    • Confirmatory testing procedures – IRMS
    • Other anabolic agents
  • Peptide Hormones, Growth Factors, Related Substances, and Mimetics
    • Erythropoietin-receptor agonists and hypoxia-inducible factor (HIF) activating agents
    • Growth hormone, its fragments and releasing factors, chorionic gonadotrophin and luteinizing hormone (LH)
  • β2‐Agonists
  • Hormone and Metabolic Modulators
  • Diuretics and other Masking Agents, Stimulants
  • Corticoids and cannabinoids
  • Manipulation of blood and blood components
  • Gene Doping
  • Conclusion


Analytical chemistry‐based research in sports drug testing has been a dynamic endeavor for several decades, with technology‐driven innovations continuously contributing to significant improvements in various regards including analytical sensitivity, comprehensiveness of target analytes, differentiation of natural/endogenous substances from structurally identical but synthetically derived compounds, assessment of alternative matrices for doping control purposes, and so forth. The resulting breadth of tools being investigated and developed by anti‐doping researchers has allowed to substantially improve anti‐doping programs and data interpretation in general. Additionally, these outcomes have been an extremely valuable pledge for routine doping controls during the unprecedented global health crisis that severely affected established sports drug testing strategies. In this edition of the annual banned‐substance review, literature on recent developments in anti‐doping published between October 2019 and September 2020 is summarized and discussed, particularly focusing on human doping controls and potential applications of new testing strategies to substances and methods of doping specified the World Anti‐Doping Agency's 2020 Prohibited List.

Original document


12 November 2020
Geyer, Hans
Kuuranne, Tiia
Thevis, Mario
Legal Terms
WADA Code, Guidelines, Protocols, Rules & Regulations
WADA Prohibited List International Standard
Other organisations
Centre Hospitalier Universitaire Vaudois (CHUV) - Lausanne University Hospital
Deutsche Sporthochschule Köln (DSHS) - German Sport University Cologne
European Monitoring Center for Emerging Doping Agents (EuMoCEDA)
Cologne, Germany: Institute of Biochemistry - German Sport University Cologne
Lausanne, Switzerland: Laboratoire Suisse d’Analyse du Dopage
Analytical aspects
Mass spectrometry analysis
Doping classes
M1. Manipulation Of Blood And Blood Components
M2. Chemical And Physical Manipulation
M3. Gene And Cell Doping
P1. Beta-Blockers
S0. Non-Approved Substances
S1. Anabolic Agents
S2. Peptide Hormones, Growth Factors
S3. Beta-2 Agonists
S4. Hormone And Metabolic Modulators
S5. Diuretics and Other Masking Agents
S6. Stimulants
S7. Narcotics
S8. Cannabinoids
S9. Glucocorticosteroids
Medical terms
Substance use research
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Scientific article
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Pdf file
Date generated
3 March 2021
Date of last modification
23 November 2022
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