Factors influencing the steroid profile in doping control analysis.

1 Jul 2008

Factors influencing the steroid profile in doping control analysis / Ute Mareck, Hans Geyer, Georg Opfermann, Mario Thevis, Wilhelm Schänzer. - (Journal of Mass Spectrometry 43 (2008) 7 (July); p. 877-891)

  • PMID: 18570179
  • DOI: 10.1002/jms.1457


Abstract

Steroid profiling is one of the most versatile and informative screening tools for the detection of steroid abuse in sports drug testing. Concentrations and ratios of various endogenously produced steroidal hormones, their precursors and metabolites including testosterone (T), epitestosterone (E), dihydrotestosterone (DHT), androsterone (And), etiocholanolone (Etio), dehydroepiandrosterone (DHEA), 5alpha-androstane-3alpha,17beta-diol (Adiol), and 5beta-androstane-3alpha,17beta-diol (Bdiol) as well as androstenedione, 6alpha-OH-androstenedione, 5beta-androstane-3alpha,17alpha-diol (17-epi-Bdiol), 5alpha-androstane-3alpha,17alpha-diol (17-epi-Adiol), 3alpha,5-cyclo-5alpha-androstan-6beta-ol-17-one (3alpha,5-cyclo), 5alpha-androstanedione (Adion), and 5beta-androstanedione (Bdion) add up to a steroid profile that is highly sensitive to applications of endogenous as well as synthetic anabolic steroids, masking agents, and bacterial activity. Hence, the knowledge of factors that do influence the steroid profile pattern is a central aspect, and pharmaceutical (application of endogenous steroids and various pharmaceutical preparations), technical (hydrolysis, derivatization, matrix), and biological (bacterial activities, enzyme side activities) issues are reviewed.

NADA Annual Report 2007 (Germany)

30 Jun 2008

Nationalen Anti Doping Agentur Deutschland (NADA) Jahresbericht 2007 / Nationale Anti Doping Agentur Deutschland (NADA). - Bonn : NADA, 2008

Essentials of Strength Training and Conditioning - third edition

30 Jun 2008

Thomas R. Baechle and Roger W. Earle (eds.). Essentials of Strength Training and Conditioning - third edition, Human Kinetics, 2008.

"Essentials of Strength and Conditioning Training", provides the latest and most comprehensive information on the structure and function of body systems, training adaptations, testing and evaluation, exercise techniques, program design, and organization and administration of facilities. It's accuracy and reliability make it not only the leading preparation resource for the CSCS-exam but also the definitive reference that strength and conditioning professionals and sports medicine specialists depend on to fine-tune their practice. Chapter objectives, key points, key terms, and self-study questions provide a structure to help readers organize and conceptualize the information.

CAS 2007_A_1394 Floyd Landis vs USADA

30 Jun 2008

CAS 2007/A/1394 Floyd Landis v. USADA

  • Cycling
  • Doping (Testosterone)
  • Presumption of compliance with applicable analysis and custodial procedures
  • Definition and construction of an International Standard for
  • Laboratories Laboratory internal chain of custody
  • ISL data recording requirements
  • Beginning of the ineligibility period

1. Pursuant to the WADA Code, there is a presumption that laboratories which have been accredited for a particular test conduct sample analysis in accordance with international laboratory standards. An athlete may rebut this presumption by establishing by a “balance of probability” that a departure from the International Standard occurred. If the athlete shows such departure, the burden then shifts to the Anti-Doping Organization to establish that such departure did not cause the Adverse Analytical Finding (AAF).

2. The Panel must take the International Standard for Laboratories (ISL) as it is written and reasonably construed and not proceed by expanding or raising the ISL and then judging the performance of an accredited laboratory by that revised more stringent standard. This is clear from the definition of an international standard found within the ISL. Proving some other alternative standard and its breach is of no consequence in attempting to rebut the presumption favouring the laboratory.

3. The ISL requires laboratories to comply with “concepts” found in the WADA Technical Documents on chain of custody, not literal compliance with it. In addition, pursuant to the WADA Technical Document on chain of custody, testimony may be used to establish chain of custody.

4. ISL 5.4.4.4.1.4 and ISL 5.2.6.1 are intended to deter reworking of data sets once produced, rather than compel laboratory technicians to produce reams of documentation in the course of analysis. So long as it is clear from the final documentation package what parameters were set, this is sufficient to guarantee that the data was not manipulated in the course of manual integration for the purpose of reaching an AAF.

5. The date of a rider’s firing from his team cannot constitute the beginning of a period of voluntary acceptance of ineligibility if, after this date and before he files a “Declaration of Voluntary Non-Competition”, he engages in legal moves that show that he does not admit to the alleged doping offence.



In July 2006 the International Cycling Union (UCI) has reported an anti-doping rule violation against the cyclist Floyd Landis after his A and B samples tested positive for the prohibited substance testosterone with a T/E ratio above the WADA threshold. Consequently he was fired from the Phonak team on 5 August 2006.

All of the A Samples from Mr. Landis' other seven samples collected during the 2006 Tour de France were tested at LNDD on the GC/MS test and resulted in a negative finding. As a result, no further testing for the B Samples was conducted.

After Mr. Landis was notified of the result of his B Sample analysis, he filed pleadings before USADA's Anti-Doping Review Board to have the case dismissed. On 18 September 2006, the Anti-Doping Review Board rejected the Appellant's petition, and the arbitration proceedings before an American Arbitration Association (AAA) Panel began.

At USADA's request and over the Appellant's objections, the AAA Panel permitted LNDD to test the B Samples of the other seven samples collected during the Tour de France using the IRMS method. LNDD found that four of the additional seven B Samples tested positive for testosterone.

After extensive pre-hearing procedures involving the determination of many complex procedural applications and following a nine day hearing held in Malibu, California, from 14 May 2007 to 23 May 2007 the AAA Panel, by its majority Award dated 20 September 2007, concluded that the charge of exogenous testosterone found in the Sample had been established in accordance with the UCI Anti-Doping Regulations.

Accordingly, the AAA Award imposed on Mr. Landis the automatic disqualification of his results in the Tour de France of 2006 and a period of two years of ineligibility running from 30 January 2007, the date of the Appellant's declaration of voluntary non-competition.

The majority Award also concluded that the charge of an elevated T/E ratio from the Sample was not established in accordance with the WADA International Standard of Laboratories.

Hereafter in October 2007 Mr. Landis appealed the AAA award with the Court of Arbitration for Sport (CAS).

The CAS Panel finds in this case that:

(i) the LNDD is a WADA-accredited laboratory which benefits from the presumption that it conducted sample analysis in accordance with international laboratory standards;
(ii) the athlete has not rebutted this presumption by showing that a departure from the International Standard occurred.

In agreement with the AAA Panel, the CAS Panel concludes that a two-year ban shall be imposed on the Appellant and that the Appellant's declaration of non-competition of 30 January 2007 constitutes voluntary acceptance of ineligibility. Accordingly, the period of ineligibility of two years shall start on that date.

Therefore the Court of Arbitration for Sport decides on 30 June 2008:

1.) The appeal filed by Mr. Floyd Landis against the award dated 20 September 2007 rendered by the AAA Panel is dismissed.

2.) Mr. Floyd Landis is ineligible to compete in cycling races for a period of two years starting from January 30, 2007.

3.) The present award is rendered without costs with the exception of the Court office fee of CHF 500 paid by the Appellant and to be retained by the CAS.

4.) The Appellant shall pay the sum of USD 100,000 to the Respondent as a contribution towards its legal fees and expenses incurred in this arbitration.

IRB 2008 IRB vs Miguel Angel Gonzalez [Spanish]

29 Jun 2008

In March 2008 the International Rugby Board (IRB) has reported an anti-doping rule violation against the Venezuelan rugby player Angel Gonzalez after his sample tested positive for the prohibited substance Nandrolone. After notification a provisional suspension was ordered and he was heard for the IRB Judicial Committee.

The Athlete accepted the test result and stated that the violation was not intentional and caused by the supplements he had used. After notification of the test result the Athlete has learned that it is not uncommon that ‘natural’ supplements contain prohibited substances and that he can’t rely solely on the label information of these supplements.

While the Athlete made reference to the lack of anti-doping education, he acknowledged that he uses the internet but had not read any of the information on the IRB website or elsewhere which describes the various anti-doping rules and programmes in Spanish.

The Judicial Committee notes, with great concern, that once again, we find ourselves dealing with an international level player who has received little, if any, anti-doping education. Although appropriate information and material is disseminated by the Board to every union, for one reason or another it seems that such information is often not disseminated onwards to players, coaches, medical advisers and others involved in the Game.

Without grounds for a reduced sanction the IRB Judicial Committee decides on 28 June 2008 to impose a 2 year period of ineligibility on the Athlete starting on the date of the provisional suspension, i.e. on 17 March 2008.

IRB 2008 IRB vs Miguel Angel Gonzalez [English]

29 Jun 2008

In March 2008 the International Rugby Board (IRB) has reported an anti-doping rule violation against the Venezuelan rugby player Angel Gonzalez after his sample tested positive for the prohibited substance Nandrolone. After notification a provisional suspension was ordered and he was heard for the IRB Judicial Committee.

The Athlete accepted the test result and stated that the violation was not intentional and caused by the supplements he had used. After notification of the test result the Athlete has learned that it is not uncommon that ‘natural’ supplements contain prohibited substances and that he can’t rely solely on the label information of these supplements.

While the Athlete made reference to the lack of anti-doping education, he acknowledged that he uses the internet but had not read any of the information on the IRB website or elsewhere which describes the various anti-doping rules and programmes in Spanish.

The Judicial Committee notes, with great concern, that once again, we find ourselves dealing with an international level player who has received little, if any, anti-doping education. Although appropriate information and material is disseminated by the Board to every union, for one reason or another it seems that such information is often not disseminated onwards to players, coaches, medical advisers and others involved in the Game.

Without grounds for a reduced sanction the IRB Judicial Committee decides on 28 June 2008 to impose a 2 year period of ineligibility on the Athlete starting on the date of the provisional suspension, i.e. on 17 March 2008.

AFLD 2008 FFHG vs Respondent M44

26 Jun 2008

Facts
The French Ice Hockey Federation (Fédération Française de Hockey sur Glace, FFHG) charges respondent M44 for a violation of the Anti-Doping Rules. During a match on December 1, 2007, a sample was taken for doping test purposes. The analysis of the sample showed the presence of a metabolite of cannabis. Cannabis is a prohibited substance according the World Anti-Doping Agency (WADA) prohibited list and is regarded as a specified substance.

History
The respondent didn't produce any information about how the prohibited substance had entered his body.

Decision
1. The sanction is a period of ineligibility of six months in which the respondent can't take part in competition or manifestations organized or authorized by the FFHG.
2. The decision will start on the date notification.
3. The decision will be published and sent to the parties involved.

AFLD 2008 FFBB vs Respondent M43

26 Jun 2008

Facts
The French Basketball Federation (Fédération Française de Basket-Ball, FFBB) charges respondent M43 or a violation of the Anti-Doping Rules. During a match on October 13, 2007, a sample was taken for doping test purposes. The analysis of the sample showed the presence of a prednisone and prednisolone. Prednisone and prednisolone are prohibited substances according the World Anti-Doping Agency (WADA) prohibited list and regarded as specified substances.

History
The respondent had medication to treat allergy symptoms of mites and pollen. He has a prescription for this medication and did mention the use of the medication on the doping control form.
However the panel considers his medical state doesn't correspond with the medication he uses.

Decision
1. The sanction is a period of ineligibility of one year, in which respondent can't take part in competition or manifestations organized or authorized by the FFBB.
2. The decision (acquittal) January 11, 2008, of the disciplinary committee of the FFBB should be modified.
3. The period of ineligibility will be reduced by the period served by the decision of January 11, 2007.
4. The decision starts on the date of notification.
5. The decision will be published and sent to the parties involved.

AFLD 2008 FFVL vs Respondent M42

26 Jun 2008

Facts
The French Free Flight Federation (Fédération Française de Vol Libre, FFVL) charges respondent M42 for a violation of the Anti-Doping Rules. During a Free Flight event on June 17, 2007, a sample for doping test purposes was taken. The analysis of the sample showed the presence of a metabolite of cannabis. Cannabis is a prohibited substance according the World Anti-Doping Agency (WADA) prohibited list and is regarded as a specified substance.

History
Respondent didn't provide any information about how the prohibited substance had entered his body.

Decision
1. The sanction is a period of ineligibility of six months in which respondent can't take part in competition or manifestations organized or authorized by the FFVL.
2. The decision starts on the date of notification.
3. The decision will be published and sent to the parties involved.

AFLD 2008 FFVL vs Respondent M41

26 Jun 2008

Facts
The French Free Flight Federation (Fédération Française de Vol Libre, FFVL) charges respondent M41 for a violation of the Anti-Doping Rules. During a Free Flight event on October 27, 2007, a sample for doping test purposes was taken. The analysis of the sample showed the presence of a metabolite of cannabis. Cannabis is a prohibited substance according the World Anti-Doping Agency (WADA) prohibited list and is regarded as a specified substance.

History
Respondent admits the use of cannabis regularly three times a week during a period of three years. There was no intention to enhance sport performance the usage was for recreational reasons only.
The panel takes in consideration the semi-professional status of the respondent and his admittance for the use of cannabis for recreational reasons.

Decision
1. The sanction is a period of ineligibility of six months in which respondent can't take part in competition or manifestations organized or authorized by the FFVL.
2. The decision starts on the date of notification.
3. The decision will be published and sent to the parties involved.

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